30,260 results

1. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIRCNpu

   Plasmid

   #74226

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Npu DnaE C-intein and Npu DnaE N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrcAgBIS (CO)

   Plasmid

   #35153

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   Bisabolene Synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6HTC_CCT3-Halo

   Plasmid

   #175322

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCT3 (CCT3 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2B-56

   Plasmid

   #177925

   Purpose

   Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   V9I; F67S; G72P, F86Y; R171K

   Promoter

   MYO2

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Use

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PARP9-c024

   Plasmid

   #173096

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   G102-K298

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBluescript-CTG-47

   Plasmid

   #99150

   Purpose

   47xCAG repeats in pBluescript vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   47xCTG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AcrIIA2

   Plasmid

   #101042

   Purpose

   Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   AcrIIA2
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAK501

    Plasmid

    #48107

    Purpose

    lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIV296

    Plasmid

    #204687

    Purpose

    AMA1 plasmid with nat1 resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Insert

    nat1
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRDX1A-c001

    Plasmid

    #175473

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX1 (PRDX1 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pWM_12x601_20bpLinker

    Plasmid

    #157787

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_20bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-HisD-mNG-Ec-Kbp-D9N

    Plasmid

    #178011

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Ec-Kbp-D9N
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK645

    Plasmid

    #71700

    Purpose

    Produces E. coli thioredoxin (EcTrxA)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNP1047

    Plasmid

    #222726

    Purpose

    pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ec dnaN-pHK/P

    Plasmid

    #237239

    Purpose

    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

    Depositor

    Michael O'Donnell

    Insert

    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    

    Use

    Tags

    his-10+PKA kinase motif+PreScission Protease clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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