30,738 results

1. pUltra-chPheRS

   Plasmid

   #182257

   Purpose

   Genetic Incorporation of DOPA

   Depositor

   Han Xiao

   Article

   Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

   Inserts

   chPheRS2 for DOPA
   chPheT for DOPA
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lac-I-SceI-Tet

   Plasmid

   #17655

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   lac I-SceI tet
   

   Expression

   Bacterial

   Available Since

   May 30, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pSpyC-GFP

   Plasmid

   #220191

   Purpose

   sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG42 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG42

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRS-LacI-dCas9

   Plasmid

   #220194

   Purpose

   mini-Tn7 dCas9 expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   LacI/pLac

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCpf1b-sp

   Plasmid

   #122188

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PT7lacO-EF-Tu-His8

   Plasmid

   #224370

   Purpose

   Express EF-Tu protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   EF-Tu (tufB E. coli)
   

   Tags

   His8

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Omega 1

   Plasmid

   #118046

   Purpose

   GoldenBraid2.0 Omega 1 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. MP4

   Plasmid

   #69652

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJYS2.1-crtYf

    Plasmid

    #89077

    Purpose

    Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYF
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE30-His-IF1

    Plasmid

    #124123

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 1 (infA Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-mH6-Cas12i2

    Plasmid

    #120883

    Purpose

    Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET mNG-GECO1

    Plasmid

    #201933

    Purpose

    Expresses mNG-GECO1 indicator in bacteria

    Depositor

    Robert Campbell

    Article

    Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

    Insert

    mNeonGreenGECO1
    

    Tags

    HIS tag

    Expression

    Bacterial

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAKgfplux3

    Plasmid

    #199304

    Purpose

    Fluorescence and bioluminescence expression in Gram-negative bacteria

    Depositor

    Attila Karsi

    Article

    Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

    Insert

    Chloramphenicol acetyltransferase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1B-GFP1-10

    Plasmid

    #193867

    Purpose

    E. coli expression vector for Hisx6 tagged GFP1-10.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    sfGFP1-10
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid

    #29657

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, NusA, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCS-mCyRFP1

    Plasmid

    #84545

    Purpose

    Bacterial expression of mCyRFP1

    Depositor

    Michael Lin

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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