32,127 results
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Plasmid#80858PurposeAccessory plasmid (encodes M13 gene VI)DepositorInsertM13 gene VI
ExpressionBacterialPromoterLambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 WT
Plasmid#13321DepositorAvailable SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 CDK2 (552)
Plasmid#61845Purposebacterial expression of GST CDK2 WTDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PARG-c002
Plasmid#173107PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNASE1_Exo-endo-phos
Plasmid#110004PurposeProtein expression and purification of DNASE1_Exo-endo-phosDepositorInsertDNASE1_Exo-endo-phos (DNASE1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
HMGCS2
Plasmid#38984PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
TagsHis and MBPExpressionBacterialAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP573
Plasmid#98796PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV WT
ExpressionBacterialMutationNoneAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HSA1_His
Plasmid#202763Purposehuman serum albumin design HSA1, in pET29b with C-terminal 6His-tagDepositorInsertHSA1
TagsHis tagExpressionBacterialPromoterT7Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
Tags6xHis and MBPExpressionBacterialAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD131
Plasmid#119886PurposeOptimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpTet_mCherry
ExpressionBacterialAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-TwStrp-FTO
Plasmid#190265PurposeExpresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b Apaf1 1-591
Plasmid#26642DepositorInsertApoptotic protease activating factor 1 (APAF1 Human)
TagsHisExpressionBacterialMutationAmino Acids 1-591Available SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAT28-proD/SaEGFP
Plasmid#120321PurposeBacterial eGFP labelingDepositorInserteGFP with Staphylococcus aureus codon optimization
ExpressionBacterialPromoterproDAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI
Plasmid#44182DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialPromoterT7Available SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
TagsHis6-TagExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET Trx His6 LIC cloning vector (2Tc-T)
Plasmid#37239DepositorTypeEmpty backboneTags6xHis and Thioredoxin (Trx)ExpressionBacterialPromoterT7Available SinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYC1446
Plasmid#158720PurposeIntegrating plasmid expressing Sth1 Cas9 and the cognate sgRNADepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P1
Plasmid#74685PurposePhlF-P1 gateDepositorInsertPTac-RBSP1-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyExpressionBacterialPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ABCE1-Halo
Plasmid#175335PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
ExpressionBacterialPromoterpBADAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only