30,738 results

1. His-SUMO-Rad23A_pET11a

   Plasmid

   #169124

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hRad23A (RAD23A Human)
   

   Expression

   Bacterial

   Mutation

   wt

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAK1

   Plasmid

   #73244

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AAK1 (AAK1 Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 47-307

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pALACE

   Plasmid

   #31853

   Depositor

   Yossef Av-Gay

   Article

   Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

   Type

   Empty backbone

   Use

   Escherichia coli-mycobacterium shuttle plasmid

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   Acetamidase operone

   Available Since

   July 9, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_AP2B1_adaptin

   Plasmid

   #178467

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   AP2B1_adaptin (AP2B1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL-2 promoter luciferase

   Plasmid

   #12194

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-2 promoter (Il2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMB67EH-YFP

   Plasmid

   #90104

   Purpose

   YFP control expression plasmid

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YFP
   

   Use

   Broad-host range

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcRho

   Plasmid

   #113121

   Purpose

   Expresses E. coli Rho

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   Rho
   

   Tags

   MGH

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5 pCOLI_A_STREP

   Plasmid

   #160147

   Purpose

   N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-human OCRL

    Plasmid

    #22207

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Oculocerebrorenal syndrome of Lowe (OCRL Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDuet1_CoiC_CoiSA

    Plasmid

    #208762

    Purpose

    Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiC
    CoiSA
    

    Expression

    Bacterial

    Mutation

    single nucleotide insertion (C70) at RBS of CoiC

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Omega 2

    Plasmid

    #118047

    Purpose

    GoldenBraid2.0 Omega 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Alpha2

    Plasmid

    #118045

    Purpose

    GoldenBraid2.0 Alpha 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-HsSENP1

    Plasmid

    #71465

    Purpose

    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRsetB-pHRed

    Plasmid

    #31472

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Tags

    7xHis

    Expression

    Bacterial

    Available Since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP1AA-c001

    Plasmid

    #53671

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1AA
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only