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32,168 results

Showing: 2721 - 2740 of 32168 results

  1. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  5. pAG GST EGFP

    Plasmid
    #236736
    Purpose
    Bacterial expression plasmid containing GST-tagged EGFP
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    EGFP
    Tags
    GST
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236736-plasmid-map-sequence-id-469209

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  15. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  17. pCDB179

    Plasmid
    #91960
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Christopher Bahl
    Type
    Empty backbone
    Tags
    10-His and Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    91960-plasmid-map-sequence-id-221552

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  19. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  20. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

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Showing: 2721 - 2740 of 32168 results