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pKT-CNP-inact

Plasmid

#211804

Purpose

Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

Depositor

Emily Weinert

Article

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Mutation

H73L H153L

Promoter

tet

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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GST PTBP1 isoform 4

Plasmid

#108594

Purpose

Recombinant GST tagged protein production

Depositor

Chris Smith

Article

Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

Insert

PTBP1 isoform 4 (PTBP1 Human)

Tags

GST

Expression

Bacterial

Mutation

isoform 4 (isoform a)

Promoter

tac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl

Plasmid

#36400

Depositor

Nora Heisterkamp

Article

ten Hoeve et al Cancer Res. 1994 May 15;54(10):2563-7.

Insert

Crkl (CRKL Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pRU1156

Plasmid

#14473

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

Gfp-mut3.1-GusA

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pBHM2641

Plasmid

#229525

Purpose

Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

Depositor

Hiten Madhani

Insert

OsTIR1

Use

CRISPR

Tags

t2a-mNeonGreen-AID*

Expression

Bacterial and Yeast

Mutation

F74G

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2644

Plasmid

#229528

Purpose

Carries AID* tag, empty sgRNA, and NATR marker

Depositor

Hiten Madhani

Insert

AID* - empty sgRNA - NATR

Tags

2xFLAG

Expression

Bacterial and Yeast

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pZH509

Plasmid

#102664

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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R701-X08-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188)

Plasmid

#159567

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_FRS2_IRS

Plasmid

#109815

Purpose

Protein expression and purification of FRS2_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

FRS2_IRS (FRS2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNP1723

Plasmid

#222727

Purpose

sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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SPS-7942

Plasmid

#80459

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEVS104

Plasmid

#207393

Purpose

Conjugal helper plasmid

Depositor

Jason Peters, Edward Ruby, Eric Stabb

Article

Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5

Plasmid

#153521

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

Depositor

Atsushi Miyawaki

Article

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(CA)5.

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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T7_MBP_TEV_ISCro4_6xHis_T7_term

Plasmid

#251502

Purpose

Bacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Bacterial

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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