30,719 results

1. pFA6a-kanMX6-P81nmt1-mECitrine

   Plasmid

   #105153

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

   Insert

   kanMX6-P81nmt1-mECitrine
   

   Tags

   81nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   81nmt1

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-kanMX6-P41nmt1-mECitrine

   Plasmid

   #105152

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

   Insert

   kanMX6-P41nmt1-mECitrine
   

   Tags

   41nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   41nmt1

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-MBP-super TEV protease

   Plasmid

   #171782

   Purpose

   Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   MBP-sTEV
   

   Tags

   Arg6 tag and Internal His6 tag

   Expression

   Bacterial

   Mutation

   No internal TEV cleavage site between the MBP and…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 His6-mEGFP-D4H

   Plasmid

   #226373

   Purpose

   Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4H (pfoA Clostridium perfringens)
   

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-EGFP-CNA35

   Plasmid

   #61603

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with EGFP

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   EGFP and CNA35
   

   Tags

   His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6-MBP-uTEV3

   Plasmid

   #135464

   Purpose

   Bacterial expression of uTEV3 (full-length) protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   His6-MBP-uTEV3
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability) , I138T/S153N…

   Promoter

   T5

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet28a_Human_H4

   Plasmid

   #42633

   Depositor

   Joe Landry

   Insert

   Human H4 (H4C1 Human)
   

   Tags

   Untagged

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG010 (DRT type 2)

    Plasmid

    #157888

    Purpose

    Expresses the DRT type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 2
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

    Plasmid

    #199248

    Purpose

    Fluorescent labelling of bacterial cells with sfGFP

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized sfGFP gene
    

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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