32,776 results
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Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneTagsLacZ'ExpressionBacterialAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
TagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOGG039
Plasmid#113996PurposeSC module. Terminator for T7RNAPDepositorInsertTerminator for T7RNAP
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pThermeleon
Plasmid#86120PurposeBandpass temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA
mWasabi
TcI38
mCherry
cI
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…PromoterpR/pL, pR/pL, pLacI, and pTlpAAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
TagstagBFPExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVHed05
Plasmid#82351PurposeContains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetRDepositorInsertsUseSynthetic BiologyExpressionBacterialAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pQE30 PTBP1 isoform1
Plasmid#108591PurposeRecombinant His tagged protein productionDepositorAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry
Plasmid#221143PurposecdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)DepositorInsertcdGreen-Matry
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
Tags6xHis-tagExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLPo
Plasmid#13792PurposeExpression of mouse codon-optimized FLP recombinaseDepositorInsertFLPo
UseCloning vectorExpressionBacterialAvailable SinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
TagsHis and MBPExpressionBacterialAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-iRFP
Plasmid#190182PurposeFluorescent reporter vector for visualization of G0 and other cell cycle phases.DepositorInsertsExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3d-E2-25K-C170S
Plasmid#18892DepositorInsertUbiquitin conjugating enzyme E2-25K (UBE2K Human, Most likely H. sapiens)
ExpressionBacterialMutationCysteine 170 is replaced with a Serine residue.Available SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1
Plasmid#218817PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinityDepositorInsertiLACCO1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.1
Plasmid#218818PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinityDepositorInsertiLACCO1.1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.2
Plasmid#218819PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinityDepositorInsertiLACCO1.2
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiLACCO1
Plasmid#218820PurposeBacterial expression of genetically encoded green fluorescent L-lactate control biosensorDepositorInsertDiLACCO1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRBS wt
Plasmid#154126PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promotor
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGEX3X-GST-PTEN
Plasmid#20743DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-GFP
Plasmid#122135PurposeGFP Reporter plasmid for B-Rex contain perfect operator sequenceDepositorInsertGFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB405-TIC21-sGFP
Plasmid#213865PurposeBinary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .DepositorInsertchloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Tt
Plasmid#154124PurposeExpresses wt Ribo-T rRNA and a set of tRNAs in bacterial cellsDepositorInsertsengineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
UseSynthetic BiologyExpressionBacterialPromoterPtac and pLAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 full-length (1–510)
Plasmid#193858PurposeExpression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationCodon optimised for expression in E. coliPromoterT7Available SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAD29_hIRG1_4-461_pvp008
Plasmid#124843PurposeExpresses human CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 462-end.PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only