Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,115 results

Showing: 5601 - 5640 of 32115 results

  3. pQE80l BCB

    Plasmid
    #196472
    Purpose
    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher
    Depositor
    Fei Sun
    Article
    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114
    Insert
    BCB
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196472-plasmid-map-sequence-id-387266

    Enlarge

  8. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

    Enlarge

  14. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

    Enlarge

  15. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

    Enlarge

  18. pDest-544

    Plasmid
    #11519
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    His6-NusA
    Expression
    Bacterial
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11519-plasmid-map-sequence-id-6969

    Enlarge

  19. pDM042

    Plasmid
    #216812
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216812-plasmid-map-sequence-id-429538

    Enlarge

  23. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

    Plasmid
    #214950
    Purpose
    Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.
    Depositor
    Dan Mulvihill
    Article
    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1
    Inserts
    VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
    VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
    Tags
    6xHis, Amino half of venus BIFC fragment, Carboxy…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    214950-plasmid-map-sequence-id-439535

    Enlarge

  31. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

    Enlarge

  32. pSCB-YFP

    Plasmid
    #101737
    Purpose
    The plasmid can be either plasmid propogation backbone or expression vector in specific strain
    Depositor
    Devaki Bhaya
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    eYFP
    Expression
    Bacterial
    Promoter
    Trc1O
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101737-plasmid-map-sequence-id-194628

    Enlarge

  38. pELxG4

    Plasmid
    #172570
    Purpose
    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-GFPmut3
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLuxB
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172570-plasmid-map-sequence-id-347356

    Enlarge

  40. pET23a-H6-TEV-cAbl

    Plasmid
    #214234
    Purpose
    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Neel Shah
    Article
    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1
    Insert
    ABL1 Kinase domain (ABL1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214234-plasmid-map-sequence-id-422815

    Enlarge

Showing: 5601 - 5640 of 32115 results