29,729 results

1. pEMJ_042

   Plasmid

   #214061

   Purpose

   T7 inducible LanTERN (pRSET)

   Depositor

   Pamela Silver

   Article

   Jones et al ACS Synth Biol. 2024 Feb 20. doi: 10.102

   Insert

   LanTERN
   

   Use

   Tags

   6x His, T7 Tag, and Xpress(tm) Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT588

   Plasmid

   #68907

   Purpose

   NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAPB02

   Plasmid

   #65628

   Purpose

   A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

   Depositor

   Pei-yuan Qian

   Article

   Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLADE(FP6*)-mCherry

   Plasmid

   #168048

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Inserts

   Blue light-inducible AraC dimers in Escherichia coli
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101* and PBAD

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p46Cpf1-OP2

   Plasmid

   #98592

   Purpose

   Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

   Depositor

   Qiong Wu

   Article

   Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   araBAD

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBintE

   Plasmid

   #48136

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR1037

   Plasmid

   #208736

   Purpose

   Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PSarA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor_Tn-pOUT

   Plasmid

   #213905

   Purpose

   Mariner transposon with outward-facing pJ23104 promoter

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and outward-facine pJ23104 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTmpKm

    Plasmid

    #176227

    Purpose

    A template plasmid for amplification of the cI-hok-neo cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, neo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-tdTomato-hphMX6

    Plasmid

    #105157

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

    Insert

    tdTomato-hphMX6
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lacI-PT5-gusA-pBAV1k

    Plasmid

    #30501

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucuronidase (gusA Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGL815

    Plasmid

    #52322

    Purpose

    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.

    Depositor

    Naweed Naqvi

    Article

    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10

    Type

    Empty backbone

    Use

    Agrobacterium

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBZS-M.CviPI

    Plasmid

    #198355

    Purpose

    Express M.CviPI in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPI (M.CviPI )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-RhoA

    Plasmid

    #12202

    Depositor

    Jonathan Chernoff

    Article

    Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZS-M.CviQIX (NDel15)

    Plasmid

    #198357

    Purpose

    Express M.CviQIX (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviQIX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbS5c-mCherry-AsLOV2*(543)

    Plasmid

    #202712

    Purpose

    Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Insert

    mCherry-AsLOV2*(543)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge