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Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
UseTagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFG036 + pFG051
Plasmid#137997PurposeTransposon mutagenesis plasmid and the cI repressor expression plasmidDepositorInsertTn5 tnp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRed pRSETB
Plasmid#159454PurposeExpresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRedDepositorInsertmCRISPRed
UseTags10 x His-TagExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mut2-RS
Plasmid#182882PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut2-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptRNA100
Plasmid#154133PurposeExpresses a set of tRNAs in bacterial cellsDepositorInserttRNAs operon under control of Ptac promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A-HAUS6_IRES_Blast
Plasmid#182886PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianMutationPromoterEF1alpha coreAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE105
Plasmid#59400PurposeMethyloBrick expression vector, tuf promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtufAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRU1099
Plasmid#14464DepositorInsertunstable gfp-mut3.1 AAV
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
A3RXB7
Plasmid#177694Purposeexpresses a N-acetyl hexosamine oxidase in E. coliDepositorInsertN-acetylhexosamine oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMal-Halo
Plasmid#209349PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotertac promoter from pKK233-2Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU424
Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-165
Plasmid#118577PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneUseTagshis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF200 Mouse beta-2 microglobulin
Plasmid#180462PurposeE. coli expression of class I MHC light chainDepositorInsertMouse beta-2 microglobulin
UseTagsBSP41ExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-xylE
Plasmid#112780PurposeEncodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.DepositorArticleInsertxylE open reading frame
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Hhe_Cas13a_WT
Plasmid#91871PurposeBacterial expression for Cas13aDepositorInsertHheCas13a (HheC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB042 (FtsZ-mEos2)
Plasmid#49764PurposeInducible expression of FtsZ-mEos2 in bacteriaDepositorInsertFtsZ-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pKD227
Plasmid#90954PurposeExpresses S. baltica TtrS under the Ptac promoterDepositorInsertTetrathionate sensor kinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.715
Plasmid#108516PurposePTac-YFP Reporter. IPTG sensor for use in Marionette strain.DepositorInsertPTac-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSW002-PpsbA_mTurquoise2
Plasmid#108237PurposeBroad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPsbA promoter - mTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-BRT17
Plasmid#45358DepositorInsert17 Repeat Beta Roll Tag
UseTagsMaltose Binding Protein (MBP)ExpressionBacterialMutationPromoterPtacAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15
Plasmid#226350PurposeExpression of proteasome substrate with tail for molecular rulerDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA030
Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsPAmCherryExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMMB67EH-dddAI
Plasmid#186559PurposeBroad host range regulated expression of the DddA immunity determinant (DddAI)DepositorInsertdddAI
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-CRM197-4xDQNAT
Plasmid#128403PurposePeriplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating G52E mutationPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only