30,260 results

1. pETduet+sfGFP-TetR

   Plasmid

   #157794

   Purpose

   Expression of sfGFP-TetR fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   sfGFP-TetR
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZac2.1 gfaABC1D-eGFP

   Plasmid

   #176861

   Purpose

   Expresses cytosolic eGFP in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-hphMX6-3nmt1

   Plasmid

   #105162

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   hphMX6-3nmt1
   

   Use

   Tags

   3nmt1

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   3nmt1

   Available since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSR1037

   Plasmid

   #208736

   Purpose

   Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PSarA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SuperNova Green/ pRSETB

   Plasmid

   #124839

   Purpose

   bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

   Insert

   SuperNova Green
   

   Use

   Tags

   No tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKM200

   Plasmid

   #13079

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   Ptac-gam-bet-exo operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPIPL12-FnCas12a

   Plasmid

   #199563

   Purpose

   Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   Fncas12a
   

   Use

   CRISPR and Synthetic Biology; Tetracycline induci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   COtetR-PIPL12

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPfetFn-Target_v2

   Plasmid

   #199564

   Purpose

   gRNA expression for FnCas12a in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   SacB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pthl_fU

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDule2-para-aminoPhe

    Plasmid

    #85503

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SIRT1.1

    Plasmid

    #13735

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pADsacA

    Plasmid

    #158649

    Purpose

    Constitutive transcription of sacA crRNA and GFP

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PT7lacO-RF1-His6

    Plasmid

    #224372

    Purpose

    Express RF1 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RF1 (prfA E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LacI-pLacI-pIV pm

    Plasmid

    #218101

    Purpose

    Phagemid that expresses LacI, and pIV under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pIV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-c4X

    Plasmid

    #75288

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGM1192

    Plasmid

    #115683

    Purpose

    E. coli-Streptomyces shuttle vector for gene expression

    Depositor

    Günther Muth

    Insert

    mCherry, aac(3)IV, tsr
    

    Use

    Tags

    Strep-tagII on mCherry, codon optimized

    Expression

    Bacterial

    Mutation

    mCherry codon optimized for Streptomyces

    Promoter

    SF14*

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FsC_pET29b

    Plasmid

    #215405

    Purpose

    Expression construct for FsC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    FsC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge