30,744 results

1. HP17deltaP8

   Plasmid

   #235447

   Purpose

   Helper plasmid for regulated phage transfer

   Depositor

   Yolanda Schaerli

   Article

   et al Nat Commun 16, 3569 (2025)

   Insert

   Coding part of the M13KO7 helper phage genome, except gene VIII
   

   Expression

   Bacterial

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW005-2

   Plasmid

   #85611

   Purpose

   Empty gRNA delivery vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH1105

   Plasmid

   #131864

   Purpose

   Produces holo-PhyB-AviTag in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

   Inserts

   PhyB(1-651)-AviTag-His6
   HO1
   PcyA
   

   Expression

   Bacterial

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-CA(A14C, E45C)

   Plasmid

   #79045

   Purpose

   Bacterial vector for cloning and propagation in DH5alpha

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   CA (A14C, E45C)
   

   Expression

   Bacterial

   Mutation

   A14C and E45C mutations in HIV-1 CA protein

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

   Plasmid

   #61582

   Purpose

   Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne2 (OTUD7A Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Isoform 2. Deleted aa 463-933.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NRASA

   Plasmid

   #25256

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NRASA:M1-N172 (NRAS Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids M1-N172

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. His6tev CBP HAT (1324-1700)

   Plasmid

   #99340

   Purpose

   bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
   

   Tags

   His6tev

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPH6

   Plasmid

   #40315

   Depositor

   Shaun Lott

   Article

   Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

   Insert

   Peptide:N-glycosidase F
   

   Tags

   6xHis and OmpA3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNP2157

    Plasmid

    #222732

    Purpose

    pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Helper (WT)
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET-28a(+)-GDH

    Plasmid

    #108954

    Purpose

    heterologous expression of GDH of B. megaterium in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Glucose dehydrogenase IV (GDH IV)
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHL 1405

    Plasmid

    #53010

    Purpose

    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp

    Expression

    Bacterial

    Mutation

    contains -56 to +141 relative to start codon

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pTOX5

    Plasmid

    #127450

    Purpose

    Encodes MqsR toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    mqsR toxin
    amilCP chromoprotein
    

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQCascade-array1

    Plasmid

    #140623

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT1-proD-mCherry

    Plasmid

    #65823

    Purpose

    Const. mCherry expression , proD promoter, E. coli Expression

    Depositor

    Michael Lynch

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    proD E . coli Constitutive Promoter

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. KLC2

    Plasmid

    #40735

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KLC2 (KLC2 Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pUA66 PcspA GFP No Linker

    Plasmid

    #105605

    Purpose

    Expresses GFP under temperature sensitive promoter PcspA

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Expression

    Bacterial

    Promoter

    PcspA

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only