32,870 results
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Plasmid#103989PurposePlasmid encoding mVenus MEDepositorInsertmVenus ME
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRExpressionBacterialAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-CHC(1-1074)-His6
Plasmid#100748PurposeBacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.DepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL003
Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
TagsHisExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-SF2
Plasmid#99020PurposeBacterial expression of SF2DepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-3WJdB-T
Plasmid#87308PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.DepositorInsertThree-way junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-Wasabi
Plasmid#86117PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38DepositorInsertsTcI38
mWasabi
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterpR/pL and pR/pL, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTcI-Wasabi
Plasmid#86101PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcIDepositorInsertsTcI
mWasabi
Tags6xHisExpressionBacterialMutationA67TPromoterpR/pL and pR/pL, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPdR
Plasmid#85083PurposeExpresses putidaredoxin reductase in E. coliDepositorInsertPutidaredoxin reductase
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZE27MC3
Plasmid#75464PurposePlasmid for constitutive expression of mcherry-pdt3DepositorInsertmcherry-pdt#3
ExpressionBacterialPromoterplacIq promoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
ExpressionBacterialPromoterchloramphenicol promoterAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseGateway CloningTags3XAU1 and 3XFLAGExpressionBacterialPromoterTRE tight promoterAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf (XSB522)
Plasmid#78512PurposeConstruct for expressing His10 - SNAPf in E. ColiDepositorInsertSNAPf
TagsHis10 followed by a prescission protease claevage…ExpressionBacterialAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Grp1-PH pGEX2TK
Plasmid#71379Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertGrp1-PH (Cyth3 Mouse)
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only