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pGEX-Cdc42

Plasmid

#12201

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NspGFP

Plasmid

#40729

Depositor

Brian McNaughton

Article

Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

Insert

N super positive GFP - Leucine Zipper

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAQ_14-3-3

Plasmid

#109943

Purpose

Protein expression and purification of YWHAQ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAQ_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11-scarREF

Plasmid

#221184

Purpose

cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen2) and PQ5 (cumate-inducible)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pScPPX2

Plasmid

#112877

Purpose

Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

Depositor

Michael Gray, Ursula Jakob

Article

Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

Insert

PPX1 (PPX1 Budding Yeast)

Tags

6X His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pet16b ALIX Bro1 (1-359)

Plasmid

#80641

Purpose

Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1, residues 1-359 (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-359

Promoter

T7

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)

Plasmid

#52054

Purpose

Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

Depositor

Jesse Rinehart

Inserts

SepRS
EF-Sep
5x tRNA Sep

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Ptac

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pRSF-FGE

Plasmid

#102615

Purpose

Inducible expression of Formylglycine-generating enzyme

Depositor

Marc Wold

Article

Bain et al Methods Enzymol. 2018;600:439-461. doi:

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmOrange

Plasmid

#37129

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

His

Expression

Bacterial

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

araB

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pYUB28b

Plasmid

#37277

Depositor

Ted Baker

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Erythropoietin

Plasmid

#182423

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-eythropoietin (EPO Human)

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET-FleBt

Plasmid

#149302

Purpose

"Enhances the yields of soluble target proteins in E. coli expression system", "Enable quick and efficient protein purification with high purity and stability"

Depositor

Michael Kolbe

Article

Wang et al PLoS Pathog. 2021 Nov 17;17(11):e1010052

Type

Empty backbone

Tags

FleBt and HRV 3C protease recognition site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1623

Plasmid

#239833

Purpose

WT OrufIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptet_P

Plasmid

#127005

Purpose

Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptet_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1346

Plasmid

#119274

Purpose

knockdown folA in K. pneumoniae

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (K. pneumoniae)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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