33,441 results

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pTW199b2

Plasmid

#136928

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII

Use

CRISPR

Expression

Bacterial

Mutation

none

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-HS

Plasmid

#133305

Purpose

"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Publication

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

hok/sok toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET-EIN-AlkBH5(66-292)

Plasmid

#125732

Purpose

Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

Depositor

Vincenzo Venditti

Publication

Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

Insert

AlkBH5(66-292) (ALKBH5 Human)

Tags

6xHis and EIN

Expression

Bacterial

Promoter

T7

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A B56α full length

Plasmid

#132635

Purpose

expresses human PP2A B56α full length in E. coli

Depositor

Wenqing Xu

Publication

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A B56α

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P-2-human RNF146 full length

Plasmid

#132610

Purpose

expresses human RNF146 full length in E. coli

Depositor

Wenqing Xu

Publication

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Publication

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)3

Plasmid

#127093

Purpose

Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Publication

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTOX10

Plasmid

#127455

Purpose

Encodes YhaV toxin. Gent-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Publication

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pB-Rex

Plasmid

#122134

Purpose

B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

Depositor

Srivatsan Raman

Publication

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

B. subtilis Rex

Expression

Bacterial

Promoter

apFAB338

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-mKelly1

Plasmid

#119782

Purpose

Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Publication

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1360

Plasmid

#119278

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-155

Plasmid

#118537

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRE-Tn7-146

Plasmid

#118562

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Publication

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMRE-Tn7-141

Plasmid

#118557

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-144

Plasmid

#118560

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Publication

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.884

Plasmid

#108536

Purpose

AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AcuRAM + PAcu-YFP

Expression

Bacterial

Mutation

AcuR: E80K R154H L202M

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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