29,771 results
-
Plasmid#73518PurposeFor recombinant expression of human GATE-16/GABARAPL2 in E. coliDepositorInsertGATE-16 (GABARAPL2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
UseTags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1 (WT)
Plasmid#208871PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsMBP-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2822
Plasmid#160670PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PUM1_PUM_pGEX
Plasmid#135129PurposeRecombinant expression of PUM1 with dual-affinity tagDepositorInsertPUM1 (PUM1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MAD2L1_HORMA
Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMAD2L1_HORMA (MAD2L1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pBbB5a-GFP
Plasmid#35352DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-K-GECO1
Plasmid#105864PurposeBacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
UseTags6-His TagExpressionBacterialMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N19_trigger
Plasmid#132747PurposeT7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNADepositorInsertToeRepG2_N19_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MP-P6
Plasmid#69665PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR dinB
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42
Plasmid#153525PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#42DepositorInsertSb#42
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.712
Plasmid#108513PurposePCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.DepositorInsertPCymRC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDM4-GLY
Plasmid#49808PurposeEncodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.DepositorInsertsphosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP5
Plasmid#75458PurposePlasmid for constitutive expression of gfp-pdt5DepositorInsertgfp-pdt#5
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_LacZ
Plasmid#139464PurposeT7 RNAP-driven expression of SNIPR RNA with a LacZ reporterDepositorInsertSNIPR_lacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B4-Leum
Plasmid#44312DepositorInsertUAS1B4-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B4-LeumAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJES103
Plasmid#135410PurposeE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporterDepositorInsertblaTEM-1
UseTagsExpressionBacterialMutationE. coli 'blaTEM-1 with M. tuberculosis mpt63…Promoterhsp60Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1C3 I0500 LasI
Plasmid#107966PurposeArabinose-inducible 3OC12HSL sender plasmidDepositorInsertlasI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pTRCHisB-TRF2∆M
Plasmid#50489PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 Delta Myb (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-437PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-T7-WspR:G249A
Plasmid#79163PurposeExpresses inactive WspR G249A under a T7 promoterDepositorInsertWspR (wspR P. areuginosa)
UseTagsExpressionBacterialMutationG249APromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK033
Plasmid#65140PurposeEncodes Venus as a Type 3 part to be used in the Dueber YTK systemDepositorInsertVenus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltalinker
Plasmid#157870Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the linkerDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltalinerPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG
Plasmid#68296PurposeS2TAG GFP reporterDepositorInsertGFP S2TAG
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
UseTagsExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-154
Plasmid#118536PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_switch
Plasmid#132742PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N02_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP
Plasmid#108410PurposeMonomeric near-infrared fluorescent protein miRFP in bacterial expression plasmidDepositorInsertmiRFP
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
UseTags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-galK
Plasmid#193727PurposeContains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galKDepositorInsertgalK (galK E. coli)
UseTagsExpressionBacterialMutationPromoterJ23113Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKEW303-AcrVA5
Plasmid#115657PurposeExpresses AcrVA5 in bacteria, under TetR control.DepositorInsertAcrVA5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30 GLTP
Plasmid#170733PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertGLTP (GLTP Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRAV23
Plasmid#67715PurposeParental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Top7 V48V
Plasmid#12393DepositorInsertTop7 V48V
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1alpha
Plasmid#51768PurposeExpresses PP1 alpha residues 7-330DepositorInsertpp1 alpha
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 alpha residues 7-330PromoterT7-lacAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
UseTagsExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only