31,059 results
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Plasmid#113315PurposeW108 scRNA.b1DepositorInsertW108 scRNA.b1_MS2
UseCRISPRExpressionBacterialAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGST-RXRalpha
Plasmid#16547DepositorInsertRXR alpha (RXRA Human)
TagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-224Available SinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pOGG024
Plasmid#113991PurposeLevel 1 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-150
Plasmid#118532PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNDGG004
Plasmid#231317PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG053
Plasmid#231322PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
ExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
TagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contai…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTP1
Plasmid#64952Purposesource of TpR FRT cassetteDepositorInsertFRT-dhfrII-FRT
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z17-ehaA_LacI
Plasmid#186313PurposePlasmid for inducible E coli expression of the Z17 helixCAM.DepositorInsertZ17-ehaA
ExpressionBacterialAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.cLT206.V5(CM2B4)
Plasmid#28189DepositorInsertMCPyV Large T antigen (206 wild-type strain)
TagsV5ExpressionBacterial and MammalianAvailable SinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
ExpressionBacterialAvailable SinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
OeNL_pRSETb
Plasmid#89529PurposeOrange color variant of bright luminescent protein for bacterial expressionDepositorInsertOeNL
TagsHis X 6ExpressionBacterialPromoterT7Available SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBL132
Plasmid#104333PurposeExpresses taz from a LacI regulated promoter under inducible IPTG control.DepositorInsertTaz
ExpressionBacterialPromoterPtacIAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJPC13
Plasmid#80773PurposeCloning vector compatible with ColE1 plasmids with medium copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CFP
Plasmid#173074PurposeExpression vector for E. coli producing target protein with N-terminal CFP fusionDepositorTypeEmpty backboneTagsmCeruleanExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR
Plasmid#173940PurposeBacterial expression of PARP1 lacking WGR domainDepositorInsertPARP1delWGR (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 521-660; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFIP
Plasmid#17565DepositorInsertinversion switch
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-UBE2S
Plasmid#66714PurposeExpresses UBE2S (1-196) in E. coliDepositorAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-MMLV-RT
Plasmid#207457Purposein vitro transcription of mRNADepositorInsertMCP-RT
ExpressionBacterialAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-193_Cacnes_HS
Plasmid#225622PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-229_Cacnes_EforCP
Plasmid#225615PurposeReplicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCPDepositorInsertEforCP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0835:Dendra
Plasmid#174923PurposeC-term fluorescent fusionDepositorInsertMSMEG_0835
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCis_2+7+8+5
Plasmid#60589Purposea 4-integrase plasmidDepositorInsertCis_2+7+8+5
ExpressionBacterialPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)
Plasmid#181789PurposeExpresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BR1.0
Plasmid#199435Purposeexpresses BAmRed1.0 in bacterial cellsDepositorInsertBAMRed
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3I3T)
Plasmid#25147PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIRT5.1
Plasmid#13738DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBV514
Plasmid#204602PurposeExpresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoterDepositorInsertOs Tir1 (LOC4335696 Oryza sativa)
TagsMyc epitope tagsExpressionBacterialMutationChanged phenylalanine 74 into an alanine (F74A).PromoterScADH1Available SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PGC1(1-400)
Plasmid#1029DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMAL_dstβL
Plasmid#73805PurposePlasmid for selection for insertion and homodimerizationDepositorInsertsCAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain
TagsPart Of the dstbl chiemra, Part of the dsTbL chim…ExpressionBacterialPromoterNaN and ToxR promotorAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
ExpressionBacterialPromoterPR promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneTagshis6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
TagsGFP-HisExpressionBacterialPromoterT7Available SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
5TTW (EED)
Plasmid#101254PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKI1
Plasmid#199118PurposeExpresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labellingDepositorInsertH6-SNAP-RapA
Tags6xHis and SNAP-tagExpressionBacterialAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET303-colE1TR
Plasmid#155181PurposeExpresses colicin E1 residues 1-364DepositorInsertcolicin E1 residues 1-364
TagsC-terminal 6x Histidine tagExpressionBacterialMutationtruncation of colicin E1 including 1-364PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
ExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
RACGAP1
Plasmid#38807PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LuxI
Plasmid#107965PurposeArabinose-inducible 3OC6HSL sender plasmidDepositorInsertluxI
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB078d3
Plasmid#79203PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah5
Plasmid#122572PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK570
Plasmid#110615PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoterDepositorInsertE2-Crimson
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-T7-SdB-T
Plasmid#87307PurposeExpresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.DepositorInsertStabilized dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only