Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 6601 - 6640 of 31059 results

  3. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    1xHA tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

    Enlarge

  17. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7 and unknown
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233296-plasmid-map-sequence-id-467796

    Enlarge

  18. pPLP4HS-mCf

    Plasmid
    #229988
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6 and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229988-plasmid-map-sequence-id-458286

    Enlarge

  19. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229984-plasmid-map-sequence-id-458288

    Enlarge

  20. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

    Enlarge

  21. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    Strep-tag II
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229986-plasmid-map-sequence-id-458306

    Enlarge

  22. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229987-plasmid-map-sequence-id-458283

    Enlarge

  24. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

    Enlarge

  35. pTrcHisA-PPARalpha(aa101-468)

    Plasmid
    #13574
    Depositor
    Martin Scanlon
    Insert
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    Tags
    x6His N-terminal, enterokinase cleavage site
    Expression
    Bacterial
    Mutation
    deleted residues 1-100.
    Available Since
    March 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    13574-plasmid-map-sequence-id-142611

    Enlarge

  38. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid
    #174692
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Jean Gariepy
    Article
    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2
    Insert
    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174692-plasmid-map-sequence-id-347066

    Enlarge

  39. pSBLego

    Plasmid
    #219967
    Purpose
    The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
    Depositor
    Markus Seeger
    Article
    Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10
    Type
    Empty backbone
    Tags
    SLEHHHHHH and pelB signal sequence
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    219967-plasmid-map-sequence-id-435554

    Enlarge

Showing: 6601 - 6640 of 31059 results