32,805 results
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Plasmid#206039PurposeHaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTagDepositorInsertTalin R3-IVVI (Tln1 Mouse)
Tags10 x His-AviTag and Halo-TagExpressionBacterialMutationThreonine 809 to Isoleucine, Threonine 833 to Val…PromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF48 HLA-A*11:01
Plasmid#180449PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*11:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK069
Plasmid#65176PurposeEncodes ConR3 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR3
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_FLAG-pvc2
Plasmid#198285PurposeContains an external epitope tag on the sheath protein; for immunofluorescence-based binding assaysDepositorInsertPVCpnf_FLAG-pvc2
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-p35
Plasmid#24895DepositorAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-VPS28
Plasmid#34803DepositorAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMycoFos
Plasmid#84577PurposeShuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-CPDBamHI-Leu
Plasmid#38256DepositorTypeEmpty backboneTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialPromoterT7Available SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF53
Plasmid#64696PurposeHis-Smt3-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, HisTag, and NpuDnaE-N102ExpressionBacterialPromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 alpha
Plasmid#24074DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-alpha-synuclein-BiFC
Plasmid#87855PurposepETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisationDepositorInsertsalpha-synuclein-VC155
alpha-synuclein-VN154
TagsC-terminal fragment of mVenus BiFC protein and N-…ExpressionBacterialPromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
TagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG419
Plasmid#115600Purposesurface-displayed Ag2; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His tagged nuclear Pif1
Plasmid#65047Purposeexpression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tagDepositorInsertYeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
TagshistagExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-LbuCas13a + crRNA negative control
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
TagsFoldon, HisTag, and SpyCatcherExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/human IL-8
Plasmid#17610DepositorInsertIL8 (CXCL8 Human)
ExpressionBacterialAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAT204
Plasmid#134881PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHC_YEAST
Plasmid#204232PurposeBacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).Available SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJS002
Plasmid#188325PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::GFP::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG014 (RADAR)
Plasmid#157892PurposeExpresses the RADAR defense systemDepositorInsertRADAR
ExpressionBacterialAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest
Plasmid#79784PurposeExpression plasmid - BsmBI entry sitesDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterpTrcAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
Tags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only