32,126 results
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Plasmid#69650PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam
ExpressionBacterialPromoterE. coli PBADAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-pBAD
Plasmid#54667PurposeLocalization: Protein Expression Vector , Excitation: 584, Emission: 607DepositorTypeEmpty backboneTags6xHis and mRFP1ExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ov-iPGM
Plasmid#180245PurposeBacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10DepositorInsertOnchocerca volvulus iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a Wb-iPGM
Plasmid#180246PurposeBacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10DepositorInsertWuchereria bancrofti iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ce-iPGM-bio
Plasmid#162559PurposeExpresses C. elegans iPGM containing a C-terminal biotinylation site and His tagDepositorInsertC. elegans iPGM bio-6His (ipgm-1 Nematode)
Tags6His tag, T7 tag, Thrombin cleavage site, and bio…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Bm-iPGM-bio
Plasmid#162565PurposeExpresses B. malayi iPGM containing a C-terminal biotinylation site and His tagDepositorInsertB. malayi iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT593
Plasmid#68909PurposeNanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEZYHb
Plasmid#18669PurposepKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteriaDepositorTypeEmpty backboneTagshemoglobinExpressionBacterialAvailable SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBSII(KSplus)-HsBGG-WT_E
Plasmid#146187PurposeBacterial Expression of HsBGG-WTDepositorInsertHsBGG-WT
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT25
Plasmid#89664Purposeexpression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT25
TagsHis6ExpressionBacterialPromoterT7Available SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT19
Plasmid#89661Purposeexpression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT19
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACAT1
Plasmid#25510PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)
Plasmid#29723DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mO…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAT205
Plasmid#134882PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ10
Plasmid#65026PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneTagslacZExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPPac-hp
Plasmid#48123PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoterDepositorInsertsignal peptide of Pac
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
ExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
Tags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
Tags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcA
Plasmid#107876PurposeExpresses E. coli RtcA in bacterial expression cellsDepositorAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG2_Actin_2
Plasmid#109990PurposeProtein expression and purification of ACTG2_Actin_2DepositorInsertACTG2_Actin_2 (ACTG2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_BauA
Plasmid#128952Purposeexpresses BauA in E.coliDepositorInsertferric acinetobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-66)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-weak
Plasmid#108314PurposeGFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterpFAB4282Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXalb_IC_Cascade
Plasmid#178765PurposeExpression of X. albilineans type I-C Cascade genesDepositorInsertX. albilineans type I-C Cascade genes
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_TS
Plasmid#140667PurposeToggle switch (TS)DepositorInsertToggle switch (TS)
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertExpressionBacterialAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-RT006-GCN4-HaloPM
Plasmid#163386PurposeTo express 4X sGCN4 module in the G-baToN systemDepositorInsert4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kindlin
Plasmid#186135PurposeFor expression of recombinant kindlin in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
ExpressionBacterialPromoterJ23107, PL(HrtO), and ProDAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMMB66EH-aphA
Plasmid#44392DepositorInsertAphA
ExpressionBacterialPromotertacAvailable SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA(D34A/D169A)
Plasmid#108693PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon, …PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
TagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only