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Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_27
Plasmid#59958PurposePTac null fragment (sigma CGG Y639A) expressionDepositorInsertnull fragment
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-IntNtr-eSrt2A
Plasmid#126015PurposeBacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-IntNtr-eSrt2A
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEN1-SGC
Plasmid#39127PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsStrep tag and SET1ExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
tIVR
Plasmid#122663PurposeExpress tIVR protein in E. coliDepositorInserttIVR
UseTagsHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TSTA3A-c013
Plasmid#53663Purposebacterial expression plasmidDepositorInsertTSTA3A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH45
Plasmid#199767PurposeExpresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH45
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorInsertRARalpha (RARA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKm
Plasmid#170331Purposebackbone vector for clone constructionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB Neo* attP
Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31TagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASR102
Plasmid#122636PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS 266-526
Plasmid#44981DepositorInsertFUS (266-526aa) (FUS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 266-526PromotertacAvailable sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PANK1
Plasmid#32871PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPANK1 (PANK1 Human)
UseTagsHis tag (with Thrombin cleavage)ExpressionBacterialMutationamino acids 231-597 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRiboI-APEX2m
Plasmid#176843PurposeExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-APEX2m
UseIntegratingTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297APromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP
Plasmid#121907PurposeExpresses BirA and eCPX-APDepositorInsertsUseTags6xHisExpressionBacterialMutationPromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorInsertDrp1 (Drp1 Fly)
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP968
Plasmid#167653PurposeExpress colicinB-His6, D17C, M261CDepositorInsertcba-his6 D17C, M261C
UseTagsExpressionBacterialMutationcba-his6 with D17C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy12
Plasmid#43977DepositorTypeEmpty backboneUseTagsOneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GEC1
Plasmid#73945PurposeFor recombinant expression of human GEC1/GABARAPL1 in E. coliDepositorInsertGEC1 (GABARAPL1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS 356-526
Plasmid#44982DepositorInsertFUS (356-526aa) (FUS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 356-526PromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(504)
Plasmid#44372DepositorInsertUAS1B8-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-TP4
Plasmid#160087PurposeBacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4DepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
UseTagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6
Plasmid#174498Purposebacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6DepositorUseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTD-DRBD
Plasmid#35622DepositorInsertPKR DRBD-1
UseTags6xHIS, HA, and TATExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWLlR5
Plasmid#149492PurposeBroad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+H1.4ΔCTD
Plasmid#157797PurposeExpression of human linker histone H1.4 without c-terminal tailDepositorInsertH1.4ΔCTD (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-sr1(SIRPT1)
Plasmid#75297PurposeExpresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 84-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBetI-E1
Plasmid#74677PurposeBetI-E1 gateDepositorInsertPTac-RBSE1-BetI-PBetI-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC012 LwCas13a locus with nontargeting spacer
Plasmid#91904PurposeLwCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLwCas13a with non-targeting spacer
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorInsertVcl (VCL Chicken)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-1~1220(FL)
Plasmid#208636Purposefor E. coli expression of human eIF5B (ENST00000289371)DepositorInserthuman eIF5B (ENST00000289371) (EIF5B Human)
UseTags6xhisExpressionBacterialMutationContain SNP rs1376443, protein sequence not chang…PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertGFP
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLIP18
Plasmid#125142PurposeGeneral purpose cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCURE
Plasmid#23013DepositorInsertI-CreI homing endonuclease
UseTagsExpressionBacterialMutationI-CreI expression is controlled by the arabinose …PromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF5
Plasmid#37219DepositorInserteIF5
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S2
Plasmid#128590PurposeExpresses tagless S2 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S2 (rpsB E.coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#14
Plasmid#153522PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#14DepositorInsertSb#14
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only