32,126 results
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Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpltetO1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJF093
Plasmid#113323PurposeSp.pCas9-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF1-02
Plasmid#24765DepositorInsertNpuDnaE-IntC
TagsGB1 and HisExpressionBacterialAvailable SinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (u-mCerulean)
Plasmid#29773DepositorTypeEmpty backboneTagsmCeruleanExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
AK1
Plasmid#39134PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
TagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9
Plasmid#137080PurposeExpression of Himar-dCas9 in bacteriaDepositorInsertHimar-dCas9
ExpressionBacterialPromoterpLtetO-1Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 vph
Plasmid#112893PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvph
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1m-ELP
Plasmid#170763PurposeEF1m laminin α1 peptide fused to I30-ELP (VPGIG)30DepositorInsertEF1m-ELP
ExpressionBacterialPromoterT7Available SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-GFP
Plasmid#220199Purposemini-Tn7 GFP expression vector - AraC/pBAD promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterAraC/pBADAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-GFP
Plasmid#220200Purposemini-Tn7 GFP expression vector - XylS/pM promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterXylS/pMAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP3666
Plasmid#222352PurposeExpression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.DepositorInsertsfGFP
ExpressionBacterialPromoterPabstBRAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP64T Activin betaB (DM#108)
Plasmid#15045DepositorInsertActivin Beta b (inhbb Frog)
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-TEVsite-human Glomulin
Plasmid#52292Purposebacterial expression of human GlomulinDepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A1_GTP-EFTU
Plasmid#109861PurposeProtein expression and purification of EEF1A1_GTP-EFTUDepositorInsertEEF1A1_GTP-EFTU (EEF1A1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
Plasmid#115214PurposeExpresses PauCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPauCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-1/2
Plasmid#91480PurposeProtein expression and purification of human SH3 domain construct GRB2-1/2DepositorInsertGRB2-1/2 (GRB2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
ExpressionBacterial and MammalianPromoterCMV, T7 lacAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTW170b2
Plasmid#136929PurposeDirected evolution of SpCas9DepositorInsertgVI
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MUNC18a
Plasmid#218888PurposeExpresses rat MUNC18a in bacteriaDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
TagsssraExpressionBacterialPromoteripaH7.8p and rpsMAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB236
Plasmid#170027PurposeEncodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM11
Plasmid#135483PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-GFPDepositorInsertSpunH2Bpr hph-GFP ADH1t
TagsGFPExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTV
Plasmid#131590PurposePlasmid for the targeting of chloramphenicol geneDepositorInsertNeomycin resistance gene
ExpressionBacterialAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchG WT
Plasmid#87107Purposeexpresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosaDepositorInsertpchG WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTAT2.2
Plasmid#35621DepositorTypeEmpty backboneTags6xHIS, T7 tag, and TATExpressionBacterialPromoterT7Available SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKW08-Lx
Plasmid#25012DepositorInsertpKW08-Lx
ExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFTC1
Plasmid#64951Purposesource of TcR FRT cassetteDepositorInsertFRT-TcR-FRT
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#221134PurposeContains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminatorDepositorInserts[PibpA-gfpASV]
[PcpxPmcherryASV] OPT
ExpressionBacterialPromotercpxP and ibpAAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterATG8Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS*11_4enh
Plasmid#149423PurposePlasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).DepositorInsertsrbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer
TagsT120>AExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_mCherry
Plasmid#187387Purposearabinose-inducible expression of mCherryDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BTK-1/1
Plasmid#91350PurposeProtein expression and purification of human SH3 domain construct BTK-1/1DepositorInsertBTK-1/1 (BTK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA
Plasmid#183027PurposeB6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy numberDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-EGFP-6H
Plasmid#187001PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only