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Plasmid#74678PurposeBM3R1-B1 gateDepositorInsertPTac-RBSB1-BM3R1-PBM3R1-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG184
Plasmid#165604PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFPDepositorInserthEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
UseSynthetic BiologyTagsExpressionBacterialMutation'CAGCG' PAM replacing the 'CGGCG…PromoterlacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MT3
Plasmid#105710PurposeBacterial expression plasmids for production of recombinant metallothionein 3DepositorInsertMetallothionein 3 (MT3 Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT4_C30Y R31W
Plasmid#105712PurposeBacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitutionDepositorInsertMetallothionein 4 (MT4 Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationThis subisoform has a C30Y and R31W substitutionPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-KSTbx2.8.2
Plasmid#42626DepositorInsertTbx2 (Tbx2 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and mGFPExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0039 DisCas7-11 only expression with CRISPR array
Plasmid#172500PurposeEncodes the DisCas7-11 protein only for bacterial expressionDepositorInsertDisCas7-11 only expression with CRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF2C-A-RFP
Plasmid#172432PurposeStrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
B7-4
Plasmid#111492PurposeMet-tRNA formyltransferaseDepositorInsertMet-tRNA formyltransferase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
UseTags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pNIC-HIS6-SUMO1-GG
Plasmid#215799PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_StefinA
Plasmid#182402PurposeBacterial expression of stefinADepositorInsertstefin (CSTA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KEAP1_KELCH
Plasmid#178496PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertKEAP1_KELCH (KEAP1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
UseTagsExpressionBacterialMutationwild typePromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pMRE160
Plasmid#118508Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2x-MT3
Plasmid#32115DepositorInsertMT (3x) (Mt1 Mouse)
UseTagsMBPExpressionBacterialMutationPromoterP-lacAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS12
Plasmid#192468PurposeBacterial expression of RvCAHS12DepositorInsertCAHS12
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFAB3677
Plasmid#47833PurposeBIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.DepositorInsertpromoter 2 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET.SUMO eIF4G1 1-200
Plasmid#158791PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.DepositorInserteIF4G1 (codon optimized)
UseTags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-UbcH10-wt
Plasmid#8503DepositorInsertUbcH10 (UBE2C Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 1, 2005AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-R273H-N268D
Plasmid#35087DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - R273H-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRTagsExpressionBacterialMutationPromoterTet promoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptdEos-CLC
Plasmid#38009DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mCherry-sfGFP
Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterCaMV 35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
WP_010880027.1
Plasmid#194729PurposeLumazine synthase of the hyperthermophile Aquifex aeolicus bacteriumDepositorInsertnanoparticle-proteinA and His tag
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11b-HA2
Plasmid#165992PurposeInfluenza HA ministem with removable HisTagDepositorInsertHA2
UseTagsFoldon and HisTagExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
UseTagsGreen fluorescent protein (GFP)ExpressionBacterialMutationPromoterfliC promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-N1IC
Plasmid#47612Purposebacterial expression of GST-myc-Notch1 ICDepositorInsertNotch1 intracellular domain (Notch1 Mouse)
UseTagsGST and mycExpressionBacterialMutationcontains amino acids 1753-2531PromotertacAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (b')
Plasmid#99837PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (b')
UseTagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3PromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA789
Plasmid#186244Purposesoc-F HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-p65(19-325)
Plasmid#44744DepositorInsertp65 (Rela Mouse)
UseTagsExpressionBacterialMutationAA 19-325PromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK004
Plasmid#171276PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPgpdA ANIA_08041 promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-Skp
Plasmid#129241PurposeProtein expression in E.coliDepositorTypeEmpty backboneUseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165489PurposeXenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseCdna expression, xenopus oocyteTagsVenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only