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31,059 results

Showing: 9451 - 9500 of 31059 results

  4. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  5. pOPINEneo-3C-GFP

    Plasmid
    #53534
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    GFP-His8
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53534-plasmid-map-sequence-id-83411

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  6. 4LG1

    Plasmid
    #60092
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL21D:JMC072-C04:C227980 (VCPKMT Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pGBW-m4046916

    Plasmid
    #145627
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145627-plasmid-map-sequence-id-292495

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  21. gNS2B47NS3 L30SF31S

    Plasmid
    #155316
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    L30 to S, F31 to S
    Promoter
    M13
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155316-plasmid-map-sequence-id-311228

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  24. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. p4286

    Plasmid
    #198152
    Purpose
    bacterial expression of sseJ::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198152-plasmid-map-sequence-id-388084

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  33. NEDD4L

    Plasmid
    #25169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    nedd4l.0574.0947 (NEDD4L Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Hect Domain
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pGEX6P1-DEST-Myc

    Plasmid
    #119752
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Myc
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119752-plasmid-map-sequence-id-233674

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  41. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  42. pCB-CBEv1

    Plasmid
    #221138
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    lacIq-Ptac
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221138-plasmid-map-sequence-id-445118

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  45. Z-VCP-10

    Plasmid
    #166559
    Purpose
    scFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166559-plasmid-map-sequence-id-329267

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  46. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    none
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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Showing: 9451 - 9500 of 31059 results