30,888 results

1. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Promoter

   Para/lac

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b-ELP[V]60

   Plasmid

   #219406

   Purpose

   (VPGVG)60

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   ELP[V]60
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJ2072.2_c1

   Plasmid

   #140675

   Purpose

   CRISPRlator control (“open ring”)

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   CRISPRlator control (“open ring”)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJLG029

   Plasmid

   #192980

   Purpose

   BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

   Depositor

   Clay Fuqua

   Article

   Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

   Insert

   pruR-3xFLAG
   

   Tags

   3XFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG8Ha-SERBP1(149-400)

   Plasmid

   #176516

   Purpose

   Expresses the human C-terminal SERBP1 149-400 construct.

   Depositor

   David Libich

   Article

   Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

   Insert

   SERBP1 149-400 (SERBP1 Human)
   

   Tags

   His8 tag, linker, TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-148 and 401-408

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2CT-Cdi

   Plasmid

   #83044

   Purpose

   Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

   Depositor

   Jennifer Doudna

   Article

   Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

   Insert

   CdiCas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEC-K-CBP_CjeCas9

   Plasmid

   #83043

   Purpose

   Expresses Campylobacter jejuni Cas9 (Cje Cas9).

   Depositor

   Jennifer Doudna

   Article

   Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

   Insert

   CjeCas9
   

   Expression

   Bacterial

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSiMPls_N + pSiMPls_C

   Plasmid

   #167203

   Purpose

   SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

   Inserts

   EGFP
   SmR 1-75 + gp41-1 N-intein
   mRuby3
   gp41-1 C-intein + SmR 76-263
   

   Expression

   Bacterial

   Promoter

   AmpR, CAT, pBAD, and pTrc

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN251

    Plasmid

    #188471

    Purpose

    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    enCas12a-HF (gift from Dr. Keith Joung)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pRE112

    Plasmid

    #43828

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET29b_mNeonGreen

    Plasmid

    #209425

    Purpose

    Express mNeonGreen in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    mNeonGreen
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-pBAD

    Plasmid

    #54762

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge