30,138 results
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Plasmid#87881PurposeE. coli expression of MKK6 aa 1-334DepositorInsertMKK6 (MAP2K6 Human)
UseTags3C protease site and 6xHisExpressionBacterialMutationaa 1-334PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-EGFP-HaloTag2
Plasmid#41742DepositorInsertHalotag 2
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
UseTagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
UseTags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-15
Plasmid#228971PurposeFor bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-15
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA36-Wasabi
Plasmid#86115PurposemWasabi reporter gene gated by TlpA36DepositorInsertsTlpA36
mWasabi
UseTags6xHisExpressionBacterialMutationD341A, P60L, D135V, K187R, K202I, L208QPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUA66 PcspA GFP No Linker
Plasmid#105605PurposeExpresses GFP under temperature sensitive promoter PcspADepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPcspAAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Emt-SH2
Plasmid#46424DepositorInsertEmt (ITK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 223-346PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a mScarlet
Plasmid#178543PurposeThe mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmScarlet
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MP4
Plasmid#69652PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only