32,161 results
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Plasmid#177293Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZDepositorInsertlacZ
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMulti_S_ccdB
Plasmid#223853PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorExpressionBacterialAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 1-143
Plasmid#108286PurposeExpresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278DepositorAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GBP-MTn
Plasmid#154363PurposeBacterial expression of MTnDepositorInsertMTn
TagsHisExpressionBacterialAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRD2
Plasmid#39074PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFloat.cspA_v1
Plasmid#133071PurposeEntry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-His6 and His6-3CExpressionBacterialPromotercspAAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39117PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK002
Plasmid#65109PurposeEncodes ConLS as a Type 1 part to be used in the Dueber YTK systemDepositorInsertConLS
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPath-Violacein-native
Plasmid#200001PurposeViolacein pathway. Native promoter and CDS sequenceDepositorInsertPathways
ExpressionBacterialAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB076i6
Plasmid#79200PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (no operator); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
ExpressionBacterialMutationR61CAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
ExpressionBacterialAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
Tags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTIII.EFGP.IGR3.6
Plasmid#40866DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_CoiC_CoiSA
Plasmid#208762PurposeTwo coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.DepositorInsertsCoiC
CoiSA
ExpressionBacterialMutationsingle nucleotide insertion (C70) at RBS of CoiCPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-BhrPETase
Plasmid#218692PurposeEncodes codon optimized BhrPETase enzyme for bacterial expressionDepositorInsertBhrPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB
Plasmid#26607DepositorAvailable SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pdestDD04Neo
Plasmid#26386DepositorTypeEmpty backboneExpressionBacterial and WormAvailable SinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1MycHis/HIP1
Plasmid#29590DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
C1-82 pComb3xss anti-cFos nanobody
Plasmid#145829PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]
Plasmid#100962PurposeContains genes encoding GPP synthase, cineole synthase and IspA(S81F)DepositorInsertGPPS-CSstr-ispA*
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB6K-mcherry-iATPsnFR1.1
Plasmid#227239PurposeInducible ATP sensor for Pseudomonas putidaDepositorInsertiATPsnFR1.1
ExpressionBacterialAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 recording plasmid
Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only