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32,811 results

Showing: 6391 - 6420 of 32811 results

  6. P25-pZE-SUMO-PbTTA

    Plasmid
    #204634
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-PbTTA
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Promoter
    PLtetO-1 promoter
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204634-plasmid-map-sequence-id-414414

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  7. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

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  8. pKL001

    Plasmid
    #98917
    Purpose
    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    CaPR
    Expression
    Bacterial
    Promoter
    BBa_J23118 - Strong Constitutive
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    98917-plasmid-map-sequence-id-189879

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  10. pAL-rfp_T

    Plasmid
    #74075
    Purpose
    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA rfp (T)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74075-plasmid-map-sequence-id-141966

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  11. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175784
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175784-plasmid-map-sequence-id-345284

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  16. PHYHD1

    Plasmid
    #38854
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHYHD1 (Phyhd1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pFnCpf1GG

    Plasmid
    #131010
    Purpose
    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of FnCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131010-plasmid-map-sequence-id-257701

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  20. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  26. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

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Showing: 6391 - 6420 of 32811 results