29,765 results

1. V72 ELP

   Plasmid

   #68938

   Purpose

   Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

   Insert

   V72 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   etfu and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE60 R124K wgMDH

   Plasmid

   #204258

   Purpose

   Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124K

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE60 R124Q wgMDH

   Plasmid

   #204259

   Purpose

   Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124Q

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE60 R124A wgMDH

   Plasmid

   #204257

   Purpose

   Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE60 R130E wgMDH

   Plasmid

   #204267

   Purpose

   Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130E

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR5pemIK

   Plasmid

   #126554

   Purpose

   Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

   Depositor

   Drake Stenger, Lindsey Burbank

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJV62

   Plasmid

   #26910

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Mol Microbiol. 2008 Mar . 67(5):1094-107

   Insert

   Che9c gene 61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCis mCherry-SS Himar1 A7

   Plasmid

   #84687

   Purpose

   Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

   Depositor

   Ulrike Munderloh

   Article

   Munderloh et al Intracellular Pathogens II: Rickettsiale

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Use

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202023

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-I48V

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK+KanaRpsL

    Plasmid

    #20871

    Depositor

    Jiyue Zhu

    Article

    Wang et al Mol Biotechnol. 2009 Jan 22. ():.

    Insert

    KanamycinResistance-RpsL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJGL002

    Plasmid

    #180606

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pet42c(+)-GST-USP5

    Plasmid

    #61750

    Purpose

    express N-terminally GST tagged human USP5

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP5 (USP5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    copFnull

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNBU2_repA2

    Plasmid

    #211911

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 6xHis(TEV)-Drp1-StrepII

    Plasmid

    #174422

    Purpose

    Expresses human Drp1 Isoform 3 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HSA2_His

    Plasmid

    #202764

    Purpose

    human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL3287

    Plasmid

    #139750

    Purpose

    Base Cas12a CRISPRi plasmid

    Depositor

    Himadri Pakrasi

    Article

    Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGLO-Magenta-AZA

    Plasmid

    #220784

    Purpose

    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    magenta chromoprotein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge