30,733 results

1. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor_Tn[himar1C9]

   Plasmid

   #213906

   Purpose

   Mariner transposon with ATc-inducible himar1C9 transposase

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-neo-sOVA

   Plasmid

   #25098

   Depositor

   Maria Castro

   Article

   Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

   Insert

   soluble ovalbumin
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. BAZ2AA-c002

   Plasmid

   #53623

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   BAZ2AA
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Brian Robertson, Peter Sander

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Expression

   Bacterial

   Promoter

   own

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pgRNA-bacteria

   Plasmid

   #44251

   Purpose

   Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA (bacteria)
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. NFAT/AP-1 3x luciferase

   Plasmid

   #11783

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   NFAT/AP-1 3x (Il2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7C-HSP90AB1

   Plasmid

   #177659

   Purpose

   Recombinant protein synthesis of human HSP90AB1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AB1 (HSP90AB1 Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Sfp

   Plasmid

   #159617

   Purpose

   Expression plasmid for Sfp phosphopantetheinyl transferase

   Depositor

   Christopher T Walsh, Jun Yin

   Article

   Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

   Insert

   Sfp
   

   Tags

   6HIS

   Expression

   Bacterial

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Expression

    Bacterial

    Promoter

    SmR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHERRY3

    Plasmid

    #24659

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-MetRS-His

    Plasmid

    #124115

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionine--tRNA ligase (metG Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-Synaptojanin 1-145

    Plasmid

    #22291

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_145 (SYNJ1 Human)
    

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Expression

    Bacterial

    Promoter

    lacIq-Ptrc

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS2513·PHP

    Plasmid

    #122590

    Purpose

    Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

    Depositor

    Pablo Ivan Nikel

    Article

    Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

    Insert

    phIorine2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pem7

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH124-ssr

    Plasmid

    #198025

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Ap/Cb resistance cassette
    

    Expression

    Bacterial

    Promoter

    ApR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD236-4b

    Plasmid

    #90956

    Purpose

    Expresses ThsS constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    ThsS
    

    Expression

    Bacterial

    Promoter

    Bba_J23104

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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