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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAT112TAG-SepT

Plasmid

#34624

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

tRNA^Sep

Expression

Bacterial

Promoter

lpp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTP434

Plasmid

#104167

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

3x Cysteines located at bps 493-495, 520-522, and…

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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HMBP-3C-STAR.66-285

Plasmid

#100094

Purpose

expresses MBP-tagged STARD1 protein domain (residues 66-285)

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

Insert

STAR protein (STAR Human)

Tags

His-tagged MBP

Expression

Bacterial

Promoter

Ptac

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Available Since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

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EHMT2

Plasmid

#25503

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

EHMT2 (EHMT2 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
SRPK1 (SRPK1A-c100)

Plasmid

#98246

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SRPK1A (SRPK1 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TccCas13a Twin SUMO

Plasmid

#199754

Purpose

Expresses codon optimized thermostabile TccCas13a in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

TccCas13a

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI2_G-alpha

Plasmid

#109791

Purpose

Protein expression and purification of GNAI2_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI2_G-alpha (GNAI2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMo168

Plasmid

#27389

Depositor

Martin Voskuil

Article

Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Insert

pMo168

Expression

Bacterial

Mutation

A replicative vector for gene expression in Burkh…

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-E1b:eGFP-Ds

Plasmid

#102417

Purpose

Fluorescent vector for screening enhancer elements in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

E1b:eGFP

Expression

Bacterial

Promoter

Zebrafish E1b minimal promoter

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-3xFLAG-LaG-2

Plasmid

#172765

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-3xFLAG-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA52_Ubiquitin

Plasmid

#110043

Purpose

Protein expression and purification of UBA52_Ubiquitin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA52_Ubiquitin (UBA52 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHV738

Plasmid

#117476

Purpose

Expression of NMT1

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAB076i7

Plasmid

#79201

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Reb_206 aka PAS629

Plasmid

#71234

Purpose

Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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