29,771 results

1. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Use

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT205

   Plasmid

   #134882

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMART-SOS-GFPuv

   Plasmid

   #102283

   Purpose

   Fluorescent sensor of E. coli SOS response

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   cda-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cda SOS promoter

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCM18

   Plasmid

   #78565

   Purpose

   60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

   Depositor

   James Kaper

   Article

   Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

   Type

   Empty backbone

   Use

   Tags

   luxCDABE

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-mDyn1 pcDNA3

   Plasmid

   #36263

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 1 (Dnm1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Type

   Empty backbone

   Use

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Use

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREDusk-AmpR-MCS

   Plasmid

   #188974

   Purpose

   For red light-repressed bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUMO2

    Plasmid

    #52259

    Purpose

    Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Bst-LF-Idaho

    Plasmid

    #170469

    Purpose

    Expresses His-tagged Bst-LF-Idaho in E. coli

    Depositor

    Jonathan Schmid-Burgk

    Insert

    Bst-LF-Idaho
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tensin-2 WT

    Plasmid

    #201797

    Purpose

    Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Use

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-GIV-CTs

    Plasmid

    #69864

    Purpose

    Express His tagged GIV-CTs (1660–1870).

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C terminal amino acids S1660-S1870 of human GIV.

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH6HTC_YBX3-Halo

    Plasmid

    #175308

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    YBX3 (YBX3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. DUSP16

    Plasmid

    #38918

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DUSP16 (DUSP16 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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