32,156 results
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Plasmid#13552DepositorInsertFLIPPi WT
TagsECFP and EYFPExpressionBacterialAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRU1098
Plasmid#14463DepositorInsertunstable gfp-mut3.1 LAA
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKEW397_AcrIIA15
Plasmid#138285PurposeExpression of AcrIIA15 proteinDepositorInsertAcrIIA15
UseCRISPRTagsMBPExpressionBacterialAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW394_AcrIIA14
Plasmid#138283PurposeExpression of AcrIIA14 proteinDepositorInsertAcrIIA14
UseCRISPRTagsSFGFPExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCM291rfp
Plasmid#59775PurposepCMrfp with TrfA mutation (291E to G)DepositorInsertrfp
ExpressionBacterialPromoterpBADAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIBB-mEGFP-IRES-puro2b
Plasmid#26462DepositorInsertpIBB
TagsmEGFPExpressionBacterial and MammalianAvailable SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP1
Plasmid#67862Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)DepositorInsertMRPP1 (TRMT10C Human)
TagsHisExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)
Plasmid#159687PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
B3-1
Plasmid#111471Purposetranslational initiation factor 2DepositorInserttranslational initiation factor 2
Tags6xHisExpressionBacterialAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-5
Plasmid#111479Purposeribosome recycling factorDepositorInsertribosome recycling factor
Tags6xHisExpressionBacterialAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
RTA
Plasmid#64029PurposeExpression of Ricin A Chain (RTA) in E. coliDepositorInsertRicin A Chain
TagsnoneExpressionBacterialAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP-GSK3
Plasmid#25133PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertcgd4_240:C29838
TagsHisExpressionBacterialAvailable SinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21c-eIF4A
Plasmid#37230DepositorAvailable SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
L-GARS-2
Plasmid#166531PurposescFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFosill-2
Plasmid#89696Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GPX3
Plasmid#38899PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
Tags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residue…PromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1005 (pSL4)
Plasmid#191000PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT5_SL
Plasmid#173769PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
TagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp1
Plasmid#156465PurposeBacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tagDepositorInsertNsp1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
P14-pZE-SUMO-ObiH
Plasmid#204629PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-ObiH
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUK
Plasmid#64185Purposeharbours marker genes URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mCeruleanBb
Plasmid#118235PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCeruleanDepositorInsertflagellin promoter driven mCerulean
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-GST
Plasmid#51528PurposeExpress GST-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT91
Plasmid#109305PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tagDepositorTypeEmpty backboneTagsSufI 1-30, 6HisExpressionBacterialPromoterT7Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltalinker
Plasmid#157870Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the linkerDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-mcherry-2a-CDK2
Plasmid#121131Purposeneutrophil specific expressionDepositorInsertdre-WT CDK2 (cdk2 Zebrafish)
Tagsmcherry-2aExpressionBacterialPromoterneutrophil specific lyzCAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-tdTomato-EC18158
Plasmid#137078PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInserttdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtPheRS
Plasmid#31080DepositorInsertMature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
TagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterT7Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT003
Plasmid#182713PurposeaTc inducible dCasRx-IF3DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 3ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSAN-QUE20C
Plasmid#129316PurposeQUEEN ATP sensor for 20degCDepositorInsertQUEEN-20C
ExpressionBacterialAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only