31,059 results
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Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneTags10-His and Smt3ExpressionBacterial and YeastPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
Tags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1dRSLE
Plasmid#13266DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #3
Plasmid#104210PurposeBacterial expression of WW domain #3 from NEDD4DepositorInsertNEDD4 WW domain #3 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.MCV.LT206.V5(CM2B4)
Plasmid#28190DepositorInsertMCPyV genomic T antigen locus (206 wild-type strain)
TagsV5ExpressionBacterial and MammalianAvailable SinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3-IR3-Bxb1-CadR-pZa
Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialPromoterInducible, HspR is its repressorAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVV098
Plasmid#215304PurposeExpresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPPhIFAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2
Plasmid#196526PurposeEntry vector for Gateway with Eps8L2DepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LasI
Plasmid#107966PurposeArabinose-inducible 3OC12HSL sender plasmidDepositorInsertlasI
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
ExpressionBacterialMutationRNA expression vector for expressing the zinc fin…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJMP1223
Plasmid#119261Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1221
Plasmid#119260Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOE6090.1
Plasmid#135080PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-Strep-tag II-eGFP
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-8+IS186
Plasmid#170636PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
ExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRE57I-GNAc
Plasmid#170637PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, GNAc cassette, lacI
ExpressionBacterialAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mVenus-I
Plasmid#163763PurposeExpresses Streptomyces codon-optimised mVenus-IDepositorInsertmVenus-I
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterSP44Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
ExpressionBacterialAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPTD-DRBD
Plasmid#35622DepositorInsertPKR DRBD-1
Tags6xHIS, HA, and TATExpressionBacterialPromoterT7Available SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046795
Plasmid#145598PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40731DepositorInsertAurora B FRET-sensor
TagsCyPet and YPetExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
DYRK2
Plasmid#39031PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb44
Plasmid#159421PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44DepositorInsertSb44
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
TagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCYP29
Plasmid#212862PurposeExpresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2x/b
Plasmid#111571PurposeExpresses a plasma membrane calcium pump hPMCA2x/bDepositorAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06_ZIKV NS5
Plasmid#145358PurposeCell-free expression vector for Zika virus NS5 protein with C-term mCherryDepositorInsertZIKV NS5
TagsmCherry and 8xHisExpressionBacterialPromoterT7Available SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWA PAS-E BAD K-GAFm
Plasmid#39866DepositorInsertsiRFP protein PAS domain
iRFP protein GAF domain (mutated)
TagsE coil and K coilExpressionBacterialMutationF165Y, D232Y, W308RPromoterarabinose inducible and rhamnose inducibleAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
L1-R184Q
Plasmid#15501DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
p3521 pET ubcH6
Plasmid#8653DepositorInsertUbcH6 (UBE2E1 Human)
ExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHYDuet183
Plasmid#41685DepositorInsertPhoRadA intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
DT049A
Plasmid#159410PurposeConstitutive murine mature BDNF expression, controlled under E.coli tet promoterDepositorInsertpr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertet promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRAV8-1
Plasmid#123375Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG84-pLas-TetRssrA
Plasmid#102455PurposeExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.DepositorAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-Pdp1
Plasmid#198320PurposeFor affinity purification of Pdp1 payloadDepositorInsertTwinStrep-SUMO-Pdp1
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only