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31,059 results

Showing: 9521 - 9560 of 31059 results

  1. PTPN11

    Plasmid
    #38965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN11 (PTPN11 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pBAD-cyScarlet

    Plasmid
    #191260
    Purpose
    Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet
    Depositor
    Robert Campbell
    Insert
    cyScarlet
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191260-plasmid-map-sequence-id-374869

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  6. SRPK2

    Plasmid
    #39047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK2 (SRPK2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Internal segment 268-518 deleted; codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. Z-UBQLN2-7

    Plasmid
    #166563
    Purpose
    scFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166563-plasmid-map-sequence-id-327818

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  8. FDX1

    Plasmid
    #39055
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FDX1 (FDX1 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  10. pCB-CBEv1

    Plasmid
    #221138
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    lacIq-Ptac
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221138-plasmid-map-sequence-id-445118

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  13. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  20. pUS252L

    Plasmid
    #191832
    Purpose
    Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuBFP
    Expression
    Bacterial
    Available Since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191832-plasmid-map-sequence-id-382125

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  34. pFP.R364

    Plasmid
    #138234
    Purpose
    Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138234-plasmid-map-sequence-id-273460

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  36. Clover2-pBAD

    Plasmid
    #54576
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and Clover2
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. RP1B-PP1gamma

    Plasmid
    #51770
    Purpose
    Expresses PP1 gamma residues 7-323
    Depositor
    Wolfgang Peti
    Insert
    pp1 gamma
    Tags
    His, TEV protease site, and Thio-6
    Expression
    Bacterial
    Mutation
    Contains PP1 gamma residues 7-323
    Promoter
    T7-lac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. mUAVbla:tetR 5′

    Plasmid
    #149379
    Purpose
    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Insert
    tetA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149379-plasmid-map-sequence-id-308791

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  39. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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Showing: 9521 - 9560 of 31059 results