31,059 results
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Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
TagsStrepIIExpressionBacterialPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2
Plasmid#124218PurposeCytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2DepositorInsertmNG-sfTq2
ExpressionBacterialAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-mad32TAG
Plasmid#188997PurposeExpress madanin-1 with TAG at 32 positionDepositorInsertmadanin-1 with TAG at 32 position
ExpressionBacterialAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-mad
Plasmid#188998PurposeExpress wildtype madanin-1DepositorInsertwildtype madanin-1
ExpressionBacterialAvailable SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEDF-PhdRS
Plasmid#127445PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348A mutations on PylRSPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GEC1
Plasmid#73945PurposeFor recombinant expression of human GEC1/GABARAPL1 in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
ExpressionBacterialAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV115
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(WT)
Plasmid#177131PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD-0016
Plasmid#12345DepositorAvailable SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB11a-GFP
Plasmid#126114PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23150Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_mmHUR-Halo
Plasmid#175311PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRCHisB-TRF2∆M
Plasmid#50489PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 Delta Myb (TERF2 Human)
Tags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-437PromoterpTrcAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZP10 Snail
Plasmid#16931DepositorInsertsnail (snai1a Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaStrp
Plasmid#67508PurposeExpress the tRNAMet scaffold with the streptavidin aptamer tagDepositorInsertmodified tRNAMet with streptavidin aptamer
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PrpoN-CroAct3-PBBa_J23106-geneIII
Plasmid#134354PurposePhagemidDepositorInsertCroAct3
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-1
Plasmid#172767PurposeBacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-1
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBP TAZ1(340-439)
Plasmid#173760Purposebacterial expression of mouse CBP TAZ1 domainDepositorAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-hphMX6
Plasmid#105159PurposeYeast gene targetingDepositorInsertGBP-hphMX6
TagsGBPExpressionBacterial and YeastAvailable SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPsrA-R1
Plasmid#74688PurposePsrA-R1 gateDepositorInsertPTac-RBSR1-PsrA-PPsrA-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S14
Plasmid#128596PurposeExpresses tagless 30S ribosomal subunit protein S14 in E.coliDepositorAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-598)
Plasmid#135437PurposeExpresses SFPQ 276-598 with TEV-cleavable hexahistidine tagDepositorInsertSplicing Factor Proline And Glutamine Rich (SFPQ Human)
TagshexahistidineExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
t1-405
Plasmid#166127PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1Head (1-405) (TLN1 Human)
TagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405 onlyAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW400hrpL-gfp
Plasmid#61437PurposepBW400hrpL-gfpDepositorInserthrpL-rbs30-gfp-ter
ExpressionBacterialPromoterHrpLAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneExpressionBacterialPromotergapDH promoter from Eggerthella lentaAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAP1LC3A
Plasmid#34824DepositorInsertmicrotubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFLxR1
Plasmid#149496PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
ExpressionBacterialMutationall charged residues are changed to S/Q except fo…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hPKM2
Plasmid#42515DepositorAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAJM.721
Plasmid#108522PurposePSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.DepositorInsertPSalTTC-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TOPOII p48 (DM#261)
Plasmid#15026DepositorInsertp48 (Ptf1a Mouse)
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only