31,059 results
-
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
ExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodA
Plasmid#89612PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase A
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Pac
Plasmid#126957PurposePromoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_cL
Plasmid#140668PurposecL control for toggle switch experimentDepositorInsertcL control for toggle switch experiment
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mTurquoise
Plasmid#103972PurposePlasmid encoding mTurquoiseDepositorInsertmTurquoise
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_266-341
Plasmid#118821PurposeBacterial expression of hnRNPA2 truncationDepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis and TEVExpressionBacterialMutationcontains amino acids 266-341Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6268 pDEST-59-His-Trx-HPV16E6
Plasmid#24126DepositorAvailable SinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE80l A-CC-A
Plasmid#196478PurposeProtein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELPDepositorInsertA-CC-A
ExpressionBacterialPromoterT7Available SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP555
Plasmid#222722PurposesfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)DepositorTypeEmpty backboneTagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC130
Plasmid#216364PurposexhNup98-Nb3i Nanobody productionDepositorInsertxhNup98-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
3TERM_bba_b0015_rev_chlo
Plasmid#163931PurposeL0 part - terminatorDepositorInsertfrom GenBank: KT364744.3, prokaryotic double terminator BBa_B0015
ExpressionBacterialAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_THRA_Hormone-recep
Plasmid#109810PurposeProtein expression and purification of THRA_Hormone-recepDepositorInsertTHRA_Hormone-recep (THRA Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_BTF3-Halo
Plasmid#175324PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1364
Plasmid#172401PurposeExpression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(WT)
Plasmid#111323Purposefor T7 transcription of wt actin pre-mRNADepositorInsertACT1
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS8: pTns(AvCAST)
Plasmid#168141PurposeInducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP120
Plasmid#71732Purposeinducible expression of TRP120 in E. coliDepositorInsertTRP120
Tags6xHIS and ThioredoxinExpressionBacterialPromoterpBADAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJES103
Plasmid#135410PurposeE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporterDepositorInsertblaTEM-1
ExpressionBacterialMutationE. coli 'blaTEM-1 with M. tuberculosis mpt63…Promoterhsp60Available SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
MP-K12
Plasmid#69656PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA dinB
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
USP21 (3MTN)
Plasmid#28112PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP21 (USP21 Human)
TagsHis tag with Thrombin cleavageExpressionBacterialMutationcatalytic domain only (amino acid residues 209-56…Available SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Trigger_27B
Plasmid#75008PurposePortion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequenceDepositorInsertZIKV_Trigger_27B
ExpressionBacterialPromoterT7Available SinceFeb. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
ExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG5271Neo
Plasmid#26387DepositorTypeEmpty backboneExpressionBacterial and WormAvailable SinceOct. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
SRSF9_2xRRM_pGEX
Plasmid#135120PurposeRecombinant expression of SRSF9 with dual-affinity tagDepositorAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23b-proMMP-7
Plasmid#29524DepositorInsertproMMP-7 (MMP7 Human)
ExpressionBacterialAvailable SinceApril 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_C1
Plasmid#171924PurposeBacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C1
TagsHis6ExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
ExpressionBacterialPromoterPxylAAvailable SinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S1
Plasmid#74691PurposeSrpR-S1 gateDepositorInsertPTac-RBSS1-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T NHE-RF
Plasmid#11636DepositorAvailable SinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Hyg)
Plasmid#110077PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-HsPABPC1_J
Plasmid#146642PurposeBacterial Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
ExpressionBacterialMutationtwo silent mutations compared to the sequence giv…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mcherry2
Plasmid#87145PurposeConstitutive E. coli mcherry Expression, with DAS4 tagDepositorInsertmcherry
TagsC terminal DAS4 tagExpressionBacterialPromoterConstitutive proDAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only