30,804 results

1. SpyCatcher002-oTet

   Plasmid

   #124663

   Purpose

   Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTet
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-mWatermelon

   Plasmid

   #190625

   Purpose

   Bacterial expression of mWatermelon GFP

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   mWatermelon
   

   Expression

   Bacterial

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINB-HOIL-1 full-length (1–510)

   Plasmid

   #193858

   Purpose

   Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

   Depositor

   Bernhard Lechtenberg

   Article

   Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

   Insert

   HOIL-1 (RBCK1 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   Codon optimised for expression in E. coli

   Promoter

   T7

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-mDyn2 pcDNA3

   Plasmid

   #36264

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 2 (Dnm2 Mouse)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pOPINK-Cezanne (OTU, aa 53-446)

   Plasmid

   #61581

   Purpose

   Expresses human Cezanne (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   Cezanne (OTUD7B Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-52 and aa 447-843.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKL11-GCaMP6f bb100

   Plasmid

   #158983

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f
   

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23100 (sigma 70)

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRD2

   Plasmid

   #39074

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD2 (BRD2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTrcHisB-TRF2

   Plasmid

   #50488

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 (TERF2 Human)
   

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-500

   Promoter

   pTrc

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-CUL4B-pCMV7.1

    Plasmid

    #155023

    Purpose

    N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 4B (CUL4B Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4Neo-murine IL-17RA-HA

    Plasmid

    #46859

    Purpose

    expresses mouse IL-17RA

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA (Il17ra Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-4T1 human PP2A B56α full length

    Plasmid

    #132635

    Purpose

    expresses human PP2A B56α full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A B56α
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbB5c-GFP

    Plasmid

    #35353

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLG028 (hhe)

    Plasmid

    #157906

    Purpose

    Expresses the hhe defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    hhe (DUF4011-helicase-Vsr-DUF3320)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAK464

    Plasmid

    #140702

    Purpose

    NBαEGFP

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαEGFP
    

    Expression

    Bacterial

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

    Plasmid

    #122084

    Purpose

    Express M.EcoGII fused to TRF1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-TERF1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of TERF1

    Expression

    Bacterial and Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

    Plasmid

    #191621

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMal-E6LLO

    Plasmid

    #51281

    Purpose

    Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

    Depositor

    Dane Wittrup

    Article

    Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

    Insert

    E6LLO
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pRSET-iSeroSnFR

    Plasmid

    #128483

    Purpose

    Fluorescent reporter for serotonin (bacterial expression)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Tags

    His, T7, and cpsfGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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