30,261 results
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Plasmid#15787DepositorInsertUbiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
human p53-(311-393)
Plasmid#24869DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains aa 311-393.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5-F
Plasmid#160081PurposeBacterial expression of C-terminally FLAG-tagged Tn5 transposaseDepositorInsertTn5 transposase
UseTagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA5 H-2K(b)
Plasmid#180441PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2K(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28c-Ku
Plasmid#161936PurposeExpresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purificationDepositorInsertku (mku Mycobacterium tuberculosis)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE100
Plasmid#59395PurposeMethyloBrick cloning vector, no promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXN_Thioredoxin
Plasmid#109778PurposeProtein expression and purification of TXN_ThioredoxinDepositorInsertTXN_Thioredoxin (TXN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-acrAB-sfGFP
Plasmid#195516PurposeInducible expression vector for transcriptionally fused acrAB and sfGFP.DepositorInsertacrAB
UseSynthetic BiologyTagssfgfpExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-YFP
Plasmid#186563Purposep15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsNoneExpressionBacterial and MammalianMutationNonePromoterPromotorlessAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-MBP
Plasmid#72323PurposeBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.DepositorInsertpET28a SnoopTag-MBP
UseTagsN-terminal His6 tag. MBP is fused at the C-termin…ExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-RalBP1
Plasmid#37245DepositorInsertRalBP1 (RALBP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-truncated
Plasmid#198286PurposeContains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCsDepositorInsertPVCpnf_pvc13-truncated
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only