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32,157 results

Showing: 10081 - 10120 of 32157 results

  14. 6ASB (FBXL19)

    Plasmid
    #119954
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    FBXL19 (FBXL19 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 31-153
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119954-plasmid-map-sequence-id-232625

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  19. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  24. pAC1750

    Plasmid
    #127984
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127984-plasmid-map-sequence-id-254510

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  28. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  29. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  34. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

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  35. DinoIII

    Plasmid
    #128100
    Purpose
    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.
    Depositor
    Senjie Lin
    Article
    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m
    Insert
    RNA complex & upstream/downstream of LHC
    Expression
    Bacterial
    Promoter
    Inserts are potential promoters
    Available Since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    128100-plasmid-map-sequence-id-292693

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  37. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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Showing: 10081 - 10120 of 32157 results