29,913 results

1. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB1H2w2-zif268

   Plasmid

   #18045

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

   Insert

   zif268
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Zif268 is fused to the omega subunit of RNA polym…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. (SUMO)10

   Plasmid

   #126948

   Purpose

   Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRMC2-tat:msfGFP

   Plasmid

   #194915

   Purpose

   Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ASB9A-c009

   Plasmid

   #53670

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   ASB9A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pECGMC3

   Plasmid

   #75441

   Purpose

   Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   mf-lon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nisA promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CYP2C9 in vector pCW ori+

   Plasmid

   #69554

   Purpose

   Modification of CYP2C9 and 2C19 s for bacterial expression

   Depositor

   Joyce Goldstein

   Insert

   CYP2C9 (CYP2C9 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG4

   Plasmid

   #70115

   Purpose

   Tn7-based lux reporter for promoter cloning in bacteria

   Depositor

   Thomas Lewis

   Article

   Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGEM cCol2 (CT#617)

   Plasmid

   #13943

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   Collagen II (COL2A1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 182-616

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pKFSS1_2

    Plasmid

    #118227

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTPRB

    Plasmid

    #39165

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRB (PTPRB Human)
    

    Use

    Tags

    GST-PreScission

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. SOCS2A-c016

    Plasmid

    #53689

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    SOCS2A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Ateam1.03-nD/nA/pRSETB

    Plasmid

    #51960

    Purpose

    bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pYC01

    Plasmid

    #224586

    Purpose

    IPTG-inducible phage φX174 lysis gene E

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    phage φX174 lysis gene E (phiX174p08 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA1lacO-1

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExp-DsbC

    Plasmid

    #112564

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbC and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPEPZ-Plac

    Plasmid

    #122635

    Purpose

    BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTEV5_ RpMatB_1

    Plasmid

    #78205

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

    Insert

    matB (His6 C106A R286C Q457C K488A)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C Q457C K488A

    Promoter

    T7

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only