30,804 results

1. pGEX-parkin WT

   Plasmid

   #45969

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (Prkn Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression

   Promoter

   tac

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET Trx His6 LIC cloning vector (2Tc-T)

   Plasmid

   #37239

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis and Thioredoxin (Trx)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pReporter_3

   Plasmid

   #60564

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_3
   

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRE-Tn5-133

   Plasmid

   #118519

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_VNp-mNeongreen-StefinA

   Plasmid

   #182419

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbS6k-RFP

   Plasmid

   #35294

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CJ8421_04975 Rptr2 recording plasmid

   Plasmid

   #194211

   Purpose

   CJ8421_04975 Rptr2 targeted DNA sequence

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   CJ8421_04975 Rptr2 DNA target
   

   Expression

   Bacterial

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hPU.1ΔN165-pET28b

   Plasmid

   #199796

   Purpose

   Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

   Depositor

   Gregory Poon

   Article

   Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

   Insert

   spi1 (SPI1 Human)
   

   Tags

   None

   Expression

   Bacterial

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nkx2.1

    Plasmid

    #15540

    Depositor

    John Rubenstein

    Article

    Shimamura et al Development. 1995 Dec . 121(12):3923-33.

    Insert

    Nkx2.1 (Nkx2-1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Aug. 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-2T-RhoA-wt

    Plasmid

    #12959

    Depositor

    Gary Bokoch

    Insert

    RhoA (RHOA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_Nsp15

    Plasmid

    #156481

    Purpose

    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-His6-Ran(Q69L)

    Plasmid

    #171563

    Purpose

    Encodes human Ran protein

    Depositor

    Yan Zeng

    Insert

    Ran (RAN Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT137-splitC

    Plasmid

    #122605

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. His SUMO TEV SPOP 1-374

    Plasmid

    #197015

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

    Insert

    SPOP (Spop Mouse)
    

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET14b – lambda exonuclease

    Plasmid

    #104531

    Purpose

    bacterial expression of lambda exonuclease

    Depositor

    Charles Bell

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

    Insert

    λ exonuclease
    

    Tags

    His6 followed by a thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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