30,261 results

1. p168-759

   Plasmid

   #62619

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule-Tet2.0

   Plasmid

   #85496

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28CLY2

   Plasmid

   #21761

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible linker containing two SGGSGG repeats
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pMXs-Runx1

   Plasmid

   #131609

   Purpose

   expression of mouse Runx1 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Runx1 (Runx1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-hXRCC1

   Plasmid

   #52274

   Purpose

   Bacterial expression of human XRCC1 with N-terminal GST-tag

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG014 (RADAR)

   Plasmid

   #157892

   Purpose

   Expresses the RADAR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   RADAR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAPK3

   Plasmid

   #73235

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 42-330

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AK2

   Plasmid

   #38829

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AK2 (AK2 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGEX6P-1-Dendra2

   Plasmid

   #82436

   Purpose

   Dendra2 expression plasmid

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

   Insert

   Dendra2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNS207

    Plasmid

    #35041

    Depositor

    Stephen Sligar

    Article

    Nikkila et al Eur J Biochem. 1991 Dec 5;202(2):309-13.

    Insert

    cytochrome b562
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TS246_pNKI-2-13_HALO-DPN7

    Plasmid

    #200720

    Purpose

    producing Methyl Tracer HALO protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    HALO-DPN7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-5X1 Flag WDR5

    Plasmid

    #15553

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Wdr5 (Wdr5 Mouse)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

14. SNX29-PX (269-391)

    Plasmid

    #119108

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX29-PX (269-391)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRExT2A-CT-BmNG

    Plasmid

    #232207

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRExT2A-CT-PmNG

    Plasmid

    #232201

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM8315

    Plasmid

    #213988

    Purpose

    PrpsM driven expression of mCherry2-L

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mCherry2-L
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet1_CylLS_CylM

    Plasmid

    #208760

    Purpose

    Expresses modified cytolysin S (mCylLS) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLS
    CylLM
    

    Use

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKESK22

    Plasmid

    #64857

    Purpose

    expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

    Depositor

    Karin Schnetz

    Article

    Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129628

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    FHL-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV,&Rhamnose

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge