32,161 results
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Plasmid#216818PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold
TagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3ExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUP1
Plasmid#223516Purposeinactive cogfp under control of Ptet, cogfp under control of PtacDepositorInsertsCoGFP
CoGFP inactivated
TagsHis tagExpressionBacterialMutationanti-dimerization mutations S129G, 22 C154S, D156…PromoterPtac, Ptac and Ptet, and PtetAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-bapaUGAc
Plasmid#201673Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensorDepositorInsertbapaUGAc
TagsHisx6ExpressionBacterialPromoteraraBADAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAB5A_Ras
Plasmid#109935PurposeProtein expression and purification of RAB5A_RasDepositorInsertRAB5A_Ras
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046740
Plasmid#145588PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX4T NHE-RF
Plasmid#11636DepositorAvailable SinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6268 pDEST-59-His-Trx-HPV16E6
Plasmid#24126DepositorAvailable SinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-(310-360)
Plasmid#24872DepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains only the dimeric oligomerization domain …Available SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S3
Plasmid#74693PurposeSrpR-S3 gateDepositorInsertPTac-RBSS3-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA8c-RFP
Plasmid#35272DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
ELF4_pet28a
Plasmid#131661PurposeBacterial expression of ETS gene ELF4DepositorAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-1
Plasmid#26077DepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S18
Plasmid#127827PurposeProduction of recombinant human ribosomal protein S18DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HGS
Plasmid#39101PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
ExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH-C234S
Plasmid#107724PurposeExpression of PHGDH C234S in bacteriaDepositorAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsNot1iso2(MCSI)_O
Plasmid#147088PurposeBacterial Expression of HsNot1iso2DepositorInsertHsNot1iso2 (CNOT1 Human)
ExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Hyg)
Plasmid#110082PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G
Plasmid#47646PurposepAC-EsaR with a a272g mutation in esaRDepositorInsertesaR-D91G
UseSynthetic BiologyExpressionBacterialMutationChanged Aspartic acid 91 to GlycinePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
ExpressionBacterialMutationwild typeAvailable SinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Hyg)
Plasmid#110077PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-C450A
Plasmid#22021PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV domain contains the light-insensitive C450A m…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
p5419
Plasmid#168186Purposebacteria reporterDepositorInsertPBAD::tag-rfp-T PtetA::sfgfp
ExpressionBacterialMutationnoneAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FSCN1-Halo
Plasmid#175343PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDH013
Plasmid#157638PurposesMHT-113 fused to GFP and RFPDepositorInsertssMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)
ExpressionBacterialAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-A1
Plasmid#114149PurposeExpresses ClbH-A1DepositorInsertclbH-A1
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationContains only the A1 domain of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-mPlum
Plasmid#117392PurposeTn7 tagging vector pTn7xTS with mPlum expression scaffoldDepositorInsertmPlum
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZP10 Snail
Plasmid#16931DepositorInsertsnail (snai1a Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX Abl-SH2
Plasmid#46402DepositorInsertAbl (ABL1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 120-225PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC301
Plasmid#31851DepositorInsertGFP (including ATG start codon)
ExpressionBacterialPromoterSODAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_switch
Plasmid#132742PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporterDepositorInsertToeRepG2_N02_switch
ExpressionBacterialAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AKAP13
Plasmid#73232PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAKAP13 (AKAP13 Human)
TagsHis-6-TEVExpressionBacterialMutationcontains amino acids 1976-2211PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
ExpressionBacterialPromoterrbcL: pBAD, chaperones: T7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only