32,812 results
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Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW170b2
Plasmid#136929PurposeDirected evolution of SpCas9DepositorInsertgVI
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-E. coli Initiation Factor 3 (IF3)
Plasmid#31340DepositorInsertE. coli Initiation Factor 3
TagsHisExpressionBacterialAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PpipB-GFPLVA
Plasmid#23344DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCC25
Plasmid#177319PurposeExpression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coliDepositorInsertTtCutA 240-603
Tags6xHis-SUMOTagExpressionBacterialPromoterT7Available SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT33
Plasmid#89668Purposeexpression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT33
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQH
Plasmid#48096PurposeCumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneTags3xFLAG and HAExpressionBacterialPromoterPQ5Available SinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H
Plasmid#118229PurposeEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA2
Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
Tags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 WT
Plasmid#67607Purposebacterial expression of wild type human PRMT9DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBL6
Plasmid#50549PurposeExpresses mCherry under the PLtetO-1 promoterDepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 1032
Plasmid#52997PurposepLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
k3(long) luciferase
Plasmid#11110DepositorAvailable SinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-43
Plasmid#225077PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII-43)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII
Plasmid#225066PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM053
Plasmid#216815PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmNeonGreen
TagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM071
Plasmid#216817PurposeAspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmCardinal
TagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
human KANK2 Ankyrin Repeats pET151
Plasmid#164054PurposeExpresses human KANK2 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterUser-definedAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRIP12-V2_A755-A841
Plasmid#194759PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJuly 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits