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pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorInsertMANF (MANF Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-pIgR cDNA
Plasmid#12109DepositorInserthuman polymeric immunoglobulin receptor (PIGR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
katushka-FMDV-P2A-pTRIP
Plasmid#163522PurposeUsed to construct lentivirus to express katushka in mammalian cells with option for a second insertDepositorInsertstopless Katushka-P2A
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBFC0687
Plasmid#186692PurposeShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargoDepositorInsertstnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBFC0625
Plasmid#186691PurposeVcDART under control of Lac promoter, Vc_lacZ_Ī±_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_Ī±_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pKM263-ScPPX
Plasmid#38327DepositorInsertPPX1 (PPX1 Budding Yeast)
UseTagsGST, His, and TEVExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/spoR
Plasmid#73631PurposeProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tagDepositorInsertguanylate kinase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
UseTagsHis tagExpressionBacterialMutationPromoterT5Available sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS0806 His14-bdSUMO-AwaIscB
Plasmid#176539PurposeBacterial expression of His14-bdSUMO-AwaIscBDepositorInsertAwaIscB
UseCRISPRTags14xHis-bdSUMO tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-135
Plasmid#118521PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alaniā¦PromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-10xCTD
Plasmid#138472PurposeExpresses GST-tagged 10xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation10x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(WT)-mCherry
Plasmid#87356PurposePI(WT): Photo-inhibitable/blue light sensitive ; WT: wild typeDepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-T7-WspR:D70E
Plasmid#79164PurposeExpresses constitutively active WspR D70E under a T7 promoterDepositorInsertWspR (wspR P. areuginosa)
UseTagsExpressionBacterialMutationD70EPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
