30,808 results

1. pIntegrase_9

   Plasmid

   #60580

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_9
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacPuroR-NeoR

   Plasmid

   #34921

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

   Inserts

   Bacterial promoter::PuroR::bacterial 3p flank
   Bacterial promoter::NeoR::bacterial 3p flank
   

   Expression

   Bacterial

   Available Since

   May 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG4

   Plasmid

   #70115

   Purpose

   Tn7-based lux reporter for promoter cloning in bacteria

   Depositor

   Thomas Lewis

   Article

   Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFA6a-PAmCherry-KanMX6

   Plasmid

   #87026

   Purpose

   Tag S. pombe genes in C terminal with PAmCherry

   Depositor

   Julien Berro

   Insert

   PAmCherry
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-MBP-NRBF2

   Plasmid

   #99330

   Purpose

   Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

   Insert

   Nuclear receptor-binding factor 2 (NRBF2 Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p168-759

   Plasmid

   #62619

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCasTns(Ptr)-IS186

   Plasmid

   #175581

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
   

   Expression

   Bacterial

   Mutation

   NO

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28aAviHisCD72cCTLD

   Plasmid

   #129066

   Purpose

   generation of biotinylated mouse CD72c CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72c (Cd72 Mouse)
   

   Expression

   Bacterial

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST17-hTRPT1-iso1

   Plasmid

   #184239

   Purpose

   For purification of N-terminally His-tagged TRPT1 from bacteria

   Depositor

   Karla Feijs

   Article

   Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

   Insert

   TRPT1 (TRPT1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRC072

    Plasmid

    #225982

    Purpose

    J23110_buj_sfGFP_buj_mRFP

    Depositor

    Jesse Zalatan

    Article

    Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

    Insert

    sfGFP (with buj RBS), mRFP (with buj RBS)
    

    Expression

    Bacterial

    Promoter

    J23110

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT593

    Plasmid

    #68909

    Purpose

    NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRE-Tn5-134

    Plasmid

    #118520

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCPP5154

    Plasmid

    #128704

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    avrPto
    

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHflu4

    Plasmid

    #203882

    Purpose

    pHI1 with insertion of monomeric red fluorescent protein (mRFP)

    Depositor

    Bogumil Karas

    Insert

    monomeric red fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF

    Plasmid

    #42519

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Expression

    Bacterial

    Promoter

    T7, T3

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. (SIM)5

    Plasmid

    #126947

    Purpose

    Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6-PRC1

    Plasmid

    #69111

    Purpose

    For bacterial expression of recombinant human PRC1 tagged with His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Protein Regulator of Cytokinesis 1 (PRC1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRC319

    Plasmid

    #61272

    Purpose

    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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