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pLVX-Tight-Puro_Nkx2.2

Plasmid

#64840

Purpose

A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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AK3L1

Plasmid

#25541

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

AK3L1 (AK4 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 25, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Nkx2.1

Plasmid

#15540

Depositor

John Rubenstein

Article

Shimamura et al Development. 1995 Dec . 121(12):3923-33.

Insert

Nkx2.1 (Nkx2-1 Mouse)

Expression

Bacterial

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only

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pE2c

Plasmid

#17462

Purpose

modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pHEA

Plasmid

#168297

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

Insert

Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)

Tags

6xHis, E-tag, and PelB signal peptide

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLG166

Plasmid

#124366

Purpose

Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Ppen

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

Plasmid

#218513

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of the Plac_mrfp cassette

Promoter

PgyrA_ermBL-ermB'

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Tags

GFP

Expression

Bacterial

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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GST-FUS-WT

Plasmid

#44978

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pTD103aiiA

Plasmid

#48884

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Danino et al Nature. 2010 Jan 21;463(7279):326-30. do

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEQ111m

Plasmid

#40190

Depositor

Paul Modrich

Article

Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

Insert

EcoRI endonuclease & EcoRI methylase

Expression

Bacterial

Mutation

changed Glutamic Acid 111 to Glutamine

Promoter

Lambda PL

Available Since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smurf1 wt

Plasmid

#13502

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pJBL3752

Plasmid

#128809

Purpose

Fluoride riboswitch-regulated sfGFP

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFTK048

Plasmid

#171320

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1-SKL fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_1353:Dendra

Plasmid

#175002

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_1353

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0250:Dendra

Plasmid

#159047

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0250

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pZP06B

Plasmid

#232321

Purpose

Make sacB a counterselection marker to replace galK

Depositor

Chenggang Wu

Insert

PrpsJ-sacB

Expression

Bacterial

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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esmGFP-C10-pVTCB052

Plasmid

#231303

Purpose

Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

Depositor

Alexander Rives

Article

Hayes et al Science. 2025 Feb 21;387(6736):850-858.

Insert

esmGFP-C10

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEXndoS

Plasmid

#44655

Depositor

Mattias Collin

Article

Collin et al Infect Immun. 2001 Nov . 69(11):7187-9.

Insert

ndoS (ndoS Streptococcus pyogenes)

Tags

Gst

Expression

Bacterial

Mutation

None

Promoter

tac

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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