32,838 results
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Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mTagBFP2
Plasmid#191746PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mTagBFP2ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJLG029
Plasmid#192980PurposeBHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purificationDepositorInsertpruR-3xFLAG
Tags3XFLAGExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE060
Plasmid#176817PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert611
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM2314-RbcS-mNG
Plasmid#165582PurposeCyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoterDepositorInsertmNeonGreen
ExpressionBacterialPromoterPrbcsAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-131
Plasmid#185923PurposeHimar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein geneDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSA12
Plasmid#186546PurposeLSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmidDepositorInsertLSSA12
Tags6xHisExpressionBacterialMutationhfYFP-G65S/Y203I/Q204E/E222D/R223FPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB213
Plasmid#101677PurposemCherry under T7 promoter controlDepositorInsertmCherry under T7 promoter control
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
ExpressionBacterialPromoterPhoQ-Ptac; lacI-PlacIqAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1003 (pSL2)
Plasmid#190998PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-cyScarlet
Plasmid#191260PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarletDepositorInsertcyScarlet
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero45
Plasmid#187931PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero45DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Synthetic, Human)
TagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorTagsGST-mCherryExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
P70a-deGFP-sc101-kan
Plasmid#184841PurposeExpression of deGFPDepositorInsertgreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterpromoter OR2-OR1Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-sfGFP
Plasmid#189675Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mEos3.2
Plasmid#189720Purposetitratable expression of mEos3.2 in bacteriaDepositorInsertmEos3.2
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-23
Plasmid#186686PurposeExpresses SNAP-23 in E. ColiDepositorInsertSNAP23
TagsGST and thrombin siteExpressionBacterialPromoterT7Available SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
TagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only