30,416 results

1. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-DogCatcher

   Plasmid

   #171772

   Purpose

   Expresses DogCatcher protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HA-AR-NTD

   Plasmid

   #171235

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORTMAGE-Ec1

   Plasmid

   #138474

   Purpose

   Enables high-efficiency recombineering in E. coli

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   CspRecT
   mutL E32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCPP5371

   Plasmid

   #128732

   Purpose

   Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

   Depositor

   Alan Collmer

   Article

   Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK793

   Plasmid

   #8827

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219V mutant
   

   Use

   Tags

   His, MBP (with TEV), and polyarginine

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability)

   Promoter

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-AviTag-DogCatcher-MBP

   Plasmid

   #171928

   Purpose

   Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogCatcher-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   DogCatcher added to N-terminus of MBP via a GSGES…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6p1-GST-ArcFL

    Plasmid

    #119877

    Purpose

    Bacterial Expression construct for protein production and purification

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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