31,059 results
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Plasmid#220783PurposeExpresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertyellow chromoprotein
Tags6xHisExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
Tags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-565
Plasmid#11520PurposeGateway destination vector for bacterial expression; His,GST tagDepositorTypeEmpty backboneUseGateway destination vectorTags6xHis and GSTExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZS-M.CviQIX (NDel15)
Plasmid#198357PurposeExpress M.CviQIX (NDel15) in bacterial cells.DepositorInsertM.CviQIX
ExpressionBacterialAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Mal-B2
Plasmid#79756PurposeSingle fluorescent protein biosensor for maltoseDepositorInsertMal-B2
Tags6xHisExpressionBacterialPromoterpTrcAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRExpressionBacterialPromoterpLtetO-1Available SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6-MBP-TEV-BirA
Plasmid#179694PurposeExpresses His6 tagged MBP-BirA fusion proteinDepositorInsertBirA
TagsHis6-MBPExpressionBacterialAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(3'')-Ia
Plasmid#112879PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ILF2_DZF_pGEX
Plasmid#135162PurposeRecombinant expression of ILF2 with dual-affinity tagDepositorAvailable SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Synthetic_Human_H2AZ.1
Plasmid#42629DepositorInsertHuman H2AZ.1 (H2AZ1 Codon Optimized, Human, Synthetic)
TagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available SinceMarch 21, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
ExpressionBacterialPromoterT7Available SinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only