30,535 results
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Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialMutationPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAZ-SK
Plasmid#73962Purposeself-killing gRNA plasmid pMAZ-SKDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBZS-YP_009702000
Plasmid#198379PurposeExpress YP_009702000 in bacterial cells.DepositorAvailable SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I-Full-length
Plasmid#210827Purposeprotein expression in ecoliDepositorInserthRIG-I (DDX58) Full length protein (RIGI Human)
UseTagsExpressionBacterialMutationNAPromoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 CDK2 (552)
Plasmid#61845Purposebacterial expression of GST CDK2 WTDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCambia1300-PacI
Plasmid#44183DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPCymRCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIOX
Plasmid#51300PurposeE.coli expression vector for His8-SUMO tagged recombinant proteinDepositorTypeEmpty backboneUseTagsHis8 + SUMOExpressionBacterialMutationPromoterT7Available SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Blue
Plasmid#220782PurposeExpresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertblue chromoprotein (aeBlue)
UseTags8xHisExpressionBacterialMutationPromoterAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFW2500
Plasmid#224213PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1 and recTDepositorInsertFncpf1 and recT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-A
Plasmid#177786PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-tag2
Plasmid#226512PurposeBacterial cell expression of SNAP-tag2DepositorInsertSNAP-tag2
UseTagsHis-tag (10x) and Strep-Tag II,ExpressionBacterialMutationPromoterAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriExRhoA2G
Plasmid#40176DepositorInsertRhoA Biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx1
Plasmid#51558PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coliDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only