29,996 results

1. S48I48 ELP

   Plasmid

   #68394

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S48I48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. T4 Lysozyme WT*

   Plasmid

   #18111

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No cysteines: C54T, C97A

   Promoter

   Available since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTOX6

   Plasmid

   #127451

   Purpose

   Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   tse2 toxin
   amilCP chromoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   pRha and tac

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST17-Cdc34a

   Plasmid

   #18674

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Cdc34A (CDC34 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-TRE3G-MYCN

   Plasmid

   #104542

   Purpose

   PiggyBac vector encoding doxycycline-inducible human MYCN

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Use

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pColdI-DDX3_helicase_core

   Plasmid

   #233618

   Purpose

   Expression of His-tagged helicase core region of DDX3X in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core WT (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-53-RtcB

    Plasmid

    #51282

    Purpose

    E. coli expression vector for E. coli RtcB RNA ligase

    Depositor

    Jay Hesselberth

    Article

    Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

    Insert

    E. coli RtcB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polymerase

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSGKP-spe

    Plasmid

    #117234

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTD103luxI_sfGFP

    Plasmid

    #48885

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI, sfGFP, and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXD71Cas10.0-Pct5.1-crRNA(AarI)

    Plasmid

    #191633

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET42b-BE3

    Plasmid

    #87437

    Purpose

    Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMH1105

    Plasmid

    #131864

    Purpose

    Produces holo-PhyB-AviTag in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

    Inserts

    PhyB(1-651)-AviTag-His6
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTOX3

    Plasmid

    #127448

    Purpose

    Encodes Tse2 toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    tse2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQE30-HyPer7

    Plasmid

    #141071

    Purpose

    Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST17-UbE2T

    Plasmid

    #15808

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2T (UBE2T Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLAM12

    Plasmid

    #26908

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Acetamidase Promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only