30,127 results

1. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT-eGFP

   Plasmid

   #215168

   Purpose

   Encodes the enhanced green fluorescent protein

   Depositor

   Travis Beddoe, Bhuvana Shanbhag

   Insert

   WT-eGFP
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWSK29

   Plasmid

   #172972

   Purpose

   Low copy number cloning vector

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTNT-B18R-6His

   Plasmid

   #58979

   Purpose

   expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Sp6

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES1L-URA

   Plasmid

   #84301

   Purpose

   E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

   Depositor

   George Church

   Article

   Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCP30T-E2Crimson

   Plasmid

   #78478

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-21a(+)-IPP1-His

   Plasmid

   #118978

   Purpose

   Bacterial expression plasmid for Inorganic pyrophosphatase

   Depositor

   Sebastian Maerkl

   Article

   Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

   Insert

   inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETx-SR6v15

    Plasmid

    #176212

    Purpose

    Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6v15
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRE145

    Plasmid

    #118497

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-mpeg1-dendra2

    Plasmid

    #51462

    Purpose

    expresses photoconvertible dendra in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

    Insert

    mpeg-dendra
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mpeg1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-PelB-6H-Ag43-160N-sfGFP

    Plasmid

    #172886

    Purpose

    Display sfGFP protein on E. coli cell surface

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    SfGFP
    Ag43, partial (flu )
    

    Use

    Tags

    HHHHHH and TEV recognition site (ENLYFQG)

    Expression

    Bacterial

    Mutation

    Deleted 1-160 (N terminal)

    Promoter

    T7 Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ1996_v2

    Plasmid

    #140664

    Purpose

    dCas9 & Csy4

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    dCas9 & Csy4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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