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Plasmid#167274PurposeOverexpression of CLIP-tag in E. coliDepositorInsertCLIP-tag
UseTagsHis x10ExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAB5AA-c003
Plasmid#175475PurposeProtein expression in bacterial cells. Entire CDS, M1-N215.DepositorInsertRAB5A (RAB5A Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial ex…PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet30b-7M SrtA
Plasmid#51141Purposeexpression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 7M
UseTags6x HisExpressionBacterialMutationP94R, E105K, E108Q, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST39-Hrs-STAM1 core
Plasmid#21497DepositorUseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
UseTagsStrep(II)-tagExpressionBacterialMutationPromoterT7 PromotorAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorInsertPTER (Pter Mouse)
UseTags6xHis and StrepExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
UseTagsExpressionBacterialMutationgfp with first three leucine codons mutated to am…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH726_pJH_LldR_PlldR-sfGFP.15TAG
Plasmid#163730PurposeLactate-inducible expression of green fluorescent protein encoding fifteen amber codonsDepositorInsertlldr gfp.15TAG
UseTagsExpressionBacterialMutationgfp with first 15 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH773_pTech_PlldR-HHRz-supP(opal)
Plasmid#163732PurposeLactate-inducible expression of supP(opal), preceded by a hammerhead ribozymeDepositorInserthhrz-supP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
UseTags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (2S-T)
Plasmid#29711DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCAT
Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/HB
Plasmid#26557DepositorInsertpT2/HB
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTD-CTwinStrep
Plasmid#45939Purposeuse to create C-terminal Twin-Strep-tag fusion proteinsDepositorTypeEmpty backboneUseTagsTwin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only