30,535 results

1. pDawn-sfGFP

   Plasmid

   #107741

   Purpose

   Expresses superfolder GFP under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLambda

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. OmpA3-PNGaseF-TEV-His6

   Plasmid

   #114274

   Purpose

   Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

   Depositor

   Martin Caffrey

   Insert

   Peptide: N-glycosidase F
   

   Use

   Tags

   OmpA3 leader sequence (for periplasmic localisati…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNeg-Ma-barnase-TAG3-TAG45

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQF

   Plasmid

   #48095

   Purpose

   Cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBC-popTag-QtE-mScarlet

   Plasmid

   #240101

   Purpose

   Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

   Depositor

   Liam Holt

   Article

   Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

   Insert

   Encapsulin
   

   Use

   Lentiviral

   Tags

   mScarlet and popZ

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UBC promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBR322-PVCpnf18-21

    Plasmid

    #198273

    Purpose

    Contains only regulatory genes; for generating empty PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf18-21
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huMb3Cas12a

    Plasmid

    #174677

    Purpose

    Expresses 6xHis-MBP tagged Mb3Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb3Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLC-217

    Plasmid

    #73163

    Purpose

    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-relE (relE E. coli (bacteria))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-sfGFP

    Plasmid

    #85482

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138128

    Purpose

    Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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