33,902 results
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Plasmid#8594DepositorAvailable sinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pEcCas-2.0
Plasmid#190697PurposeConstitutive expressing of Cas9 and inducible expression of lambda RedDepositorInsertCas9 and Lamda Red system
ExpressionBacterialAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
Tags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
ExpressionBacterialPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorPublicationInsertLgBiT-PhoCl1-SmBiT-MBP
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPP537
Plasmid#158803PurposepRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
Tags2xSV40NLS-6xHisExpressionBacterialAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP1
Plasmid#129499PurposeExpresses AausFP1 constitutively in E. coli (most strains)DepositorInsertAausFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-QUE2m
Plasmid#129350PurposeATP measurementDepositorInsertQUEEN-2m
ExpressionBacterialAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUS252
Plasmid#127674PurposeExpresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbonesDepositorInsertFree Use GFP (fuGFP)
ExpressionBacterialPromoterlacAvailable sinceJune 24, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCF525-EF1a-Hygro-P2A-mCherry-lenti
Plasmid#115796PurposeExpresses mCherry in human cells, contained in a lentiviral vector.DepositorInsertmCherry
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
ExpressionBacterialAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-IG-mSA
Plasmid#80706PurposeExpresses monomeric streptavidin (mSA) in bacterial cellsDepositorInsertMonomeric streptavidin
Tags10xHis tag and TEV siteExpressionBacterialPromoterT7Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by