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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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31,662 results

Showing: 1241 - 1260 of 31662 results

  1. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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  2. T4 Lysozyme WT*

    Plasmid
    #18111
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    No cysteines: C54T, C97A
    Available Since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18111-plasmid-map-sequence-id-174143

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  5. mu-GM-CSF-pCIneo

    Plasmid
    #163518
    Purpose
    Expresses murine GM-CSF in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine GM-CSF
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163518-plasmid-map-sequence-id-321579

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  7. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  8. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  13. pAIP4_5F66

    Plasmid
    #234226
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5F66 (PPIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234226-plasmid-map-sequence-id-464191

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  14. Dox-SOScat

    Plasmid
    #207329
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    son of sevenless homolog 2 variant, catalytic domain
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 593 to 1078
    Promoter
    Tet Response Element
    Available Since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207329-plasmid-map-sequence-id-413071

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  17. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11518-plasmid-map-sequence-id-181065

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  20. PYCR1

    Plasmid
    #38842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PYCR1 (PYCR1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1260 of 31662 results