30,535 results

1. BirA in pET28a (w400-2)

   Plasmid

   #26624

   Depositor

   Eric Campeau

   Insert

   BirA
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMRE-Tn7-151

   Plasmid

   #118565

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOSIP-TT (TetR, P21)

   Plasmid

   #45989

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX_hDDX5_FL_wt

   Plasmid

   #88871

   Purpose

   inducible expression of GST-DDX5 in bacteria

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EcAvs4

   Plasmid

   #188794

   Purpose

   Avs4 from Escherichia coli NCTC11132

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tdTomato-pBAD

   Plasmid

   #54856

   Purpose

   Localization: Protein Expression Vector , Excitation: 554, Emission: 581

   Depositor

   Michael Davidson, Nathan Shaner, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and tdTomato

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDFDuet-1::ADAT3-ADAT2

   Plasmid

   #15917

   Depositor

   Nina Papavasiliou

   Article

   Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

   Insert

   ADAT3 & ADAT2
   

   Use

   Tags

   FLAG and HexaHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-T7-Ct-YhjH

    Plasmid

    #79167

    Purpose

    Dual expression of Ct biosensor and YhjH under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    yhjH
    T7-Spinach2-Ct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YARS_pNIC-CTB10H

    Plasmid

    #153059

    Purpose

    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    YARS (YARS1 Human)
    

    Use

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pALACE

    Plasmid

    #31853

    Depositor

    Yossef Av-Gay

    Article

    Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

    Type

    Empty backbone

    Use

    Escherichia coli-mycobacterium shuttle plasmid

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Acetamidase operone

    Available Since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

17. EcRho

    Plasmid

    #113121

    Purpose

    Expresses E. coli Rho

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    Rho
    

    Use

    Tags

    MGH

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Omega 2

    Plasmid

    #118047

    Purpose

    GoldenBraid2.0 Omega 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Alpha2

    Plasmid

    #118045

    Purpose

    GoldenBraid2.0 Alpha 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP1AA-c001

    Plasmid

    #53671

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1AA
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only