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pET24a-VHH-std

Plasmid

#109417

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDB016

Plasmid

#99220

Purpose

Accessory plasmid for aaRS PACE; negative selection

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII-neg

Expression

Bacterial

Promoter

PT7

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Publication

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Tags

His tag

Expression

Bacterial

Promoter

TRC

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET_sfCherry(1-10)_32aalinker_sfCherry(11)

Plasmid

#83030

Purpose

Bacterial expression vector for split sfCherry screening

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry(1-10)_32aalinker_sfCherry(11)

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Publication

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pMAL-c4X

Plasmid

#75288

Purpose

Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pComb3XLambda

Plasmid

#63892

Purpose

Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

human antibody lambda light chain (IGLL5 Human)

Use

Phage display

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Publication

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Publication

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pRSETa_ mEos4a

Plasmid

#51072

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant

Depositor

Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4a

Expression

Bacterial

Promoter

T7

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTD-CTwinStrep

Plasmid

#45939

Purpose

use to create C-terminal Twin-Strep-tag fusion proteins

Depositor

Thorben Dammeyer

Publication

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Publication

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Publication

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pACYC-T7

Plasmid

#41187

Depositor

Dan Bolon

Publication

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUNIV-EGFP

Plasmid

#24706

Depositor

Cynthia Czajkowski

Publication

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP (eGFP Aequoria victoria)

Expression

Bacterial, Mammalian, and Y…

Available since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNIC-CH

Plasmid

#26117

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Type

Empty backbone

Tags

His

Expression

Bacterial

Promoter

T7-lacO

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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