30,260 results

1. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Peter Sander, Brian Robertson

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   own

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. mRuby2-pBAD

   Plasmid

   #54771

   Purpose

   Localization: Protein Expression Vector , Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   6xHis and mRuby2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SARS-CoV-2_N_FL

   Plasmid

   #157867

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPYtag-Histag-Avidin

   Plasmid

   #214836

   Purpose

   This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-Histag-Avidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNPTS138 Cm

   Plasmid

   #41891

   Depositor

   Howard Steinman

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDR111_Bgal no NLS

   Plasmid

   #188397

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Phyper spank

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-SpyCatcher002

   Plasmid

   #102827

   Purpose

   Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pGDP2 MCR-1

    Plasmid

    #118404

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    MCR-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-28c S2

    Plasmid

    #171920

    Purpose

    Express RNA-based (p)ppGpp sensor

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Insert

    RNA-based (p)ppGpp sensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSP1E2

    Plasmid

    #20063

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E2 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE-60NA-msGFP2

    Plasmid

    #135301

    Purpose

    Encodes a photostable monomeric superfolder GFP

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    msGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge