31,022 results

1. pAB035h

   Plasmid

   #79191

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 gIII, luxAB; Ptet rpoZ-HA4
   

   Expression

   Bacterial

   Promoter

   Plac62; Ptet

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB021CH(+)

   Plasmid

   #99209

   Purpose

   Accessory plasmid for PACE of PylRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   PT7 [SD8] gIII, luxAB; ProK pylT
   

   Expression

   Bacterial

   Promoter

   PT7 and PproK

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-1-Skp2 WT

   Plasmid

   #81117

   Purpose

   bacterial expression plasmid of Skp2

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   SKP2 (SKP2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

   Plasmid

   #114626

   Purpose

   Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   P90C; 43 polyQ repeat

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. cBEST4

   Plasmid

   #234660

   Purpose

   Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

   Depositor

   Mingzi M. Zhang

   Article

   Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

   Inserts

   spCas9 cytosine base editor
   Golden Gate compatible sgRNA insertion cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3 and tcp830

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. NW30

   Plasmid

   #133444

   Purpose

   Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW30
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEVOL-pBpF

    Plasmid

    #31190

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.

    Depositor

    Peter Schultz

    Article

    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

    Insert

    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    

    Expression

    Bacterial

    Mutation

    Y32G, E107P, D158T, I159S

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTOX6

    Plasmid

    #127451

    Purpose

    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    tse2 toxin
    amilCP chromoprotein
    

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SBFP2-anti-AlfaTag nanobody

    Plasmid

    #171826

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    SBFP2-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. MBP-Benzonase

    Plasmid

    #218192

    Purpose

    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

    Depositor

    Cheryl Arrowsmith

    Insert

    nucA
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    April 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198467

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMV306hsp+LuxG13

    Plasmid

    #26161

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

    Plasmid

    #199605

    Purpose

    Expression of cell permeable opAsCas12a in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    opCas12a-T8N
    

    Use

    CRISPR

    Tags

    6xHis, Twin-strep, SUMO

    Expression

    Bacterial

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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