30,733 results

1. pD454-SR rat Alpha-synuclein

   Plasmid

   #89074

   Purpose

   bacterial expression of rat Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Rat)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-MHL: nsp10_SARS-CoV-2

   Plasmid

   #167851

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp10

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pT7-7 asyn A30P

   Plasmid

   #105726

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   A30P

   Promoter

   T7

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 28A_APEX2_MSP1D1

   Plasmid

   #170923

   Purpose

   Express APEX2_MSP1D1 in Bl21

   Depositor

   Huan Bao

   Article

   Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

   Insert

   APEX2-MSP
   

   Expression

   Bacterial

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-7 asyn G51D

   Plasmid

   #105747

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

   Insert

   alpha-synuclein G51D (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   G51D

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINE-3C-mCherry

   Plasmid

   #214060

   Purpose

   Expression of fluorescent fusion proteins

   Depositor

   Ray Owens

   Insert

   mCherry
   

   Tags

   Hexahistidine

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD434-SR mouse Alpha-synuclein

   Plasmid

   #89073

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS mouse IRS-2

    Plasmid

    #11372

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Expression

    Bacterial

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP4639

    Plasmid

    #107771

    Purpose

    Expression of EYFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EYFP in pME6010
    

    Tags

    pLacZ-EYFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5VBD (USP09X)

    Plasmid

    #101260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP09X (USP9X Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pT7-7 asyn S129A

    Plasmid

    #36048

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S129A

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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