30,808 results

1. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

   Plasmid

   #198751

   Purpose

   Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - LargeBiT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6_BcLOV4_mCherry_BamUK

   Plasmid

   #114596

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Tags

   His6 and mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. U_(pos36GFP)-Cys

   Plasmid

   #71758

   Purpose

   U_+36GFP-Cys fusion expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   U_+36GFP-Cys
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH625_pJH_LldR_PlldR-sfGFP.0TAG

   Plasmid

   #163724

   Purpose

   Lactate-inducible expression of green fluorescent protein encoding zero amber codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyBADNT

   Plasmid

   #84690

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   AraC

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbA5k-EPL123

   Plasmid

   #45361

   Depositor

   Aindrila Mukhopadhyay

   Article

   Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

   Insert

   AcrAB
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Bacterial

   Promoter

   LacUV5

   Available Since

   June 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF-G1(5CNW)RS

   Plasmid

   #222872

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVS104

   Plasmid

   #207393

   Purpose

   Conjugal helper plasmid

   Depositor

   Jason Peters, Edward Ruby, Eric Stabb

   Article

   Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK1126

    Plasmid

    #236187

    Purpose

    BsaI compatible gentamicin resistance cassette

    Depositor

    Jeffrey Barrick

    Article

    VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

    Insert

    gentamicin resistance cassette
    

    Expression

    Bacterial

    Promoter

    CP25

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTXB1-pA-M.EcoGII

    Plasmid

    #192873

    Purpose

    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

    Depositor

    Chong Tang

    Article

    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

    Insert

    pA-M.EcoGII
    

    Tags

    3X Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. prhaBAD-anti-rabbit-Nb-Hia5

    Plasmid

    #239627

    Purpose

    Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

    Insert

    Hia5
    

    Tags

    6HIS, MBP, TEV, and anti-Rabbit Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. TDP-43_CTD

    Plasmid

    #98669

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Promoter

    T5-LacO

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHyo245

    Plasmid

    #173147

    Purpose

    Expresses pheS_A294G by dual T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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