31,022 results
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Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneTags6xhisExpressionBacterialPromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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pBS aP2 promoter (5.4kb) polyA
Plasmid#11424DepositorInsertaP2 promoter (Fabp4 Mouse)
ExpressionBacterialAvailable SinceApril 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTE102
Plasmid#59397PurposeMethyloBrick expression vector, mxaF promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPmxaFAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
TagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b Apaf1 1-591
Plasmid#26642DepositorInsertApoptotic protease activating factor 1 (APAF1 Human)
TagsHisExpressionBacterialMutationAmino Acids 1-591Available SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
ExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-p35
Plasmid#24895DepositorAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBLind
Plasmid#113109PurposeVector encoding the RFP gene under blue light-inducible promoter BindF1.DepositorInsertRFP
UseCell-freeExpressionBacterialPromoterBindF1Available SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyExpressionBacterialMutationL401GPromoterPalkSAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE105
Plasmid#59400PurposeMethyloBrick expression vector, tuf promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPtufAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
Tags6x His Tag, GFP, and TEV TagExpressionBacterialPromoterT5 promoter/lac operator elementAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR
Plasmid#109055PurposeBacterial expression of E.coli DHFR proteinDepositorInsertecDHFR
ExpressionBacterialPromoterT7Available SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(His10-PD-1cyto)
Plasmid#177896PurposeE coli expression of His10 tag fused to human PD-1 Intracellular DomainDepositorInsertPD-1 (aa 194-288 only) (PDCD1 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GARTA
Plasmid#42388PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ALFA-TwinStrep
Plasmid#240252PurposeIPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tagsDepositorTypeEmpty backboneTags10x HIS, ALFA, Thrombin, and Twin-StrepExpressionBacterialPromoterT7Available SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_IRF3_SMAD/FHA
Plasmid#178479PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
Tags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only