31,022 results

1. pBAD-ApplePy1Highest

   Plasmid

   #239054

   Purpose

   Bacterial expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   ApplePy1Highest
   

   Expression

   Bacterial

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-ApplePy1Low

   Plasmid

   #239056

   Purpose

   Bacterial expression of low affinity red fluorescent pyruvate biosensor ApplePy1Low

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   ApplePy1Low
   

   Expression

   Bacterial

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-ApplePy1High

   Plasmid

   #239055

   Purpose

   Bacterial expression of high affinity red fluorescent pyruvate biosensor ApplePy1High

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   ApplePy1High
   

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-dApplePy1

   Plasmid

   #239058

   Purpose

   Bacterial expression of red fluorescent pyruvate control biosensor dApplePy1

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   dApplePy1
   

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB602-OEP7-mVenus

   Plasmid

   #213864

   Purpose

   Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

   Insert

   chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
   

   Expression

   Bacterial and Plant

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB1-mGFPmut2

   Plasmid

   #103980

   Purpose

   Plasmid encoding mGFPmut2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mGFPmut2
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S65A, V68L, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATetO 6XHis T5 Exonuclease

    Plasmid

    #206209

    Purpose

    Inducible expression of T5 exonuclease

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    T5 exonuclease
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGLO-Blue

    Plasmid

    #220782

    Purpose

    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    blue chromoprotein (aeBlue)
    

    Tags

    8xHis

    Expression

    Bacterial

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HMA-APOBEC1-Nb

    Plasmid

    #226012

    Purpose

    For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

    Depositor

    Eugene Yeo

    Article

    Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Tags

    His-MBP and antiRabbit-nanobody

    Expression

    Bacterial

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid

    #160730

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Expression

    Bacterial

    Promoter

    Rhamnose promoter

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK302

    Plasmid

    #87768

    Purpose

    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Inserts

    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli …

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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