30,808 results

1. pFN18A-HaloTag-Biotin

   Plasmid

   #206039

   Purpose

   HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Insert

   Talin R3-IVVI (Tln1 Mouse)
   

   Tags

   10 x His-AviTag and Halo-Tag

   Expression

   Bacterial

   Mutation

   Threonine 809 to Isoleucine, Threonine 833 to Val…

   Promoter

   T7

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBCPB+

   Plasmid

   #18940

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   attP, lacZ, attB
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHelper_TetR/pTet-Tnpase (camR)

   Plasmid

   #213910

   Purpose

   ATc-inducible himar1C9 transposase expression - cam resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES002 MBP-GSK3β_S9A-HA-His

   Plasmid

   #196184

   Purpose

   Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Insert

   GSK3B (GSK3B Human)
   

   Tags

   HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM643

   Plasmid

   #112533

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Expression

   Bacterial

   Mutation

   A17P

   Promoter

   J23107, PL(HrtO), and ProD

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB405-TIC21-sGFP

   Plasmid

   #213865

   Purpose

   Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

   Insert

   chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
   

   Expression

   Bacterial and Plant

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTn7-SCOUT11

    Plasmid

    #200991

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. (SUMO)10-(SIM)5

    Plasmid

    #126866

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid

    #176534

    Purpose

    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associated IscB (Delaware Bay metagenome)
    

    Tags

    14xHis-MBP-bdSUMO and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGMβ1

    Plasmid

    #216202

    Purpose

    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

    Depositor

    Yasuko Sasaki

    Article

    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

    Type

    Empty backbone

    Use

    Shuttle vector, conjugal plasmid, theta type rep…

    Expression

    Bacterial

    Promoter

    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

    Available Since

    Jan. 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBad-HisB-GRvT

    Plasmid

    #87363

    Purpose

    Expresses GRvT, a tandem heterodimer green and red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GRvT
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    AraBad

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJUMP18-Uac

    Plasmid

    #126959

    Purpose

    Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTn7-SCOUT13

    Plasmid

    #200993

    Purpose

    pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTn7-SCOUT12

    Plasmid

    #200992

    Purpose

    pLv1-pUC18R6KT-miniTn7-Km, KmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. rpoDs366C

    Plasmid

    #129688

    Purpose

    Expression of Sigma 70 S366C

    Depositor

    Jeff Gelles

    Article

    Mumm et al bioRxiv 2020

    Insert

    rpoD (rpoD E. coli)
    

    Tags

    His6/thrombin

    Expression

    Bacterial

    Mutation

    C132S C291S C295S S366C

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SRM

    Plasmid

    #25490

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SRM (SRM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pBbS8k-RFP_R43W_E93G

    Plasmid

    #109406

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R43W and E93G repA mutations to increase copy num…

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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