30,882 results

1. pTNT-B18R

   Plasmid

   #58978

   Purpose

   expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Expression

   Bacterial

   Promoter

   T7/Sp6

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_TIA1

   Plasmid

   #106095

   Purpose

   bacterial protein expression of TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   TIA1 (TIA1 Human)
   

   Tags

   6xHIs-thrombin and T7

   Expression

   Bacterial

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. DP6

   Plasmid

   #140446

   Purpose

   Untargetted bacterial drift

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 gIII
   

   Expression

   Bacterial

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-E12-vioABECD

   Plasmid

   #66537

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Tags

   6X His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Expression

    Bacterial

    Promoter

    AmpR and lpp

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-mE1

    Plasmid

    #32534

    Depositor

    Jorge Eduardo Azevedo

    Article

    Carvalho et al Mol Biotechnol. 2011 Oct 20.

    Insert

    E1, ubiquitin-activating enzyme (Uba1 Mouse)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138130

    Purpose

    Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-mouse-IgG(kappa)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWM_12x601_20bpLinker

    Plasmid

    #157787

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_20bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL99

    Plasmid

    #78577

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Expression

    Bacterial

    Promoter

    inducible PnitA-NitR system

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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