31,022 results

1. pET28-H12TEV

   Plasmid

   #208635

   Purpose

   for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS aP2 promoter (5.4kb) polyA

   Plasmid

   #11424

   Depositor

   Ronald Kahn

   Insert

   aP2 promoter (Fabp4 Mouse)
   

   Expression

   Bacterial

   Available Since

   April 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE102

   Plasmid

   #59397

   Purpose

   MethyloBrick expression vector, mxaF promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PmxaF

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pALOD4 non-binding mutant

   Plasmid

   #111027

   Purpose

   Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A, G501A, T502A, T503A, L5…

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b Apaf1 1-591

   Plasmid

   #26642

   Depositor

   Guy Salvesen

   Article

   Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

   Insert

   Apoptotic protease activating factor 1 (APAF1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acids 1-591

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pETM6-At4CL-CmCHS-MsCHI

   Plasmid

   #73394

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   CmCHS
   MsCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-p35

   Plasmid

   #24895

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

   Insert

   p35 (CDK5R1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Full-length p35

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pBLind

   Plasmid

   #113109

   Purpose

   Vector encoding the RFP gene under blue light-inducible promoter BindF1.

   Depositor

   Chueh Loo Poh

   Article

   Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

   Insert

   RFP
   

   Use

   Cell-free

   Expression

   Bacterial

   Promoter

   BindF1

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIBA3-SpyCatcher-sfGFP

   Plasmid

   #107420

   Purpose

   For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   SpyCatcher
   

   Tags

   His tag, StrepII, and superfolder GFP

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK01_PalkS_alkS

    Plasmid

    #166502

    Purpose

    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    alkS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    L401G

    Promoter

    PalkS

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE105

    Plasmid

    #59400

    Purpose

    MethyloBrick expression vector, tuf promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptuf

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Promoter

    T5 promoter/lac operator element

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-ecDHFR

    Plasmid

    #109055

    Purpose

    Bacterial expression of E.coli DHFR protein

    Depositor

    Stephen Benkovic

    Article

    Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GARTA

    Plasmid

    #42388

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GARTA (GART Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET15b-ALFA-TwinStrep

    Plasmid

    #240252

    Purpose

    IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

    Depositor

    Olga Boudker, Krishna Reddy

    Article

    Reddy et al Nat Struct Mol Biol (2025).

    Type

    Empty backbone

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_IRF3_SMAD/FHA

    Plasmid

    #178479

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    IRF3_IRF3 (IRF3 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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