31,008 results

1. SpyCatcher002-oPent

   Plasmid

   #124664

   Purpose

   Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oPent
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-N2H-N1

   Plasmid

   #125547

   Purpose

   Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 attached to n-terminus of Gatew…

   Expression

   Bacterial and Mammalian

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-jRCaMP1a

   Plasmid

   #113936

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jRCaMP1a
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher002-oHept

   Plasmid

   #124671

   Purpose

   Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHept
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH773_pTech_PlldR-HHRz-supP(opal)

   Plasmid

   #163732

   Purpose

   Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   hhrz-supP
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23a-HsRPS27a

   Plasmid

   #69561

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. His-GIV-CTs

   Plasmid

   #69864

   Purpose

   Express His tagged GIV-CTs (1660–1870).

   Depositor

   Pradipta Ghosh

   Article

   Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

   Insert

   GIV/Girdin (CCDC88A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C terminal amino acids S1660-S1870 of human GIV.

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 803 pGEX2T PTEN

   Plasmid

   #10784

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pKEW336-MBP-TEV-MbCas12a-33362

   Plasmid

   #115670

   Purpose

   Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   MbCas12a
   

   Use

   CRISPR

   Tags

   MBP-TEV

   Expression

   Bacterial

   Promoter

   T7 RNAP

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKEN GFP mut3

    Plasmid

    #20410

    Depositor

    Stanley Falkow, Raphael Valdivia

    Article

    Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

    Insert

    GFP
    

    Expression

    Bacterial

    Mutation

    S65G S72A

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pKEW321-MbCas12a-RFP-GFP-array

    Plasmid

    #115661

    Purpose

    Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    GFP and RFP array for MbCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180155

    Purpose

    td-sfGFP cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MLH1

    Plasmid

    #28120

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-347.

    Available Since

    March 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pKOS6b-KOpstB

    Plasmid

    #237501

    Purpose

    Knockout of pstB

    Depositor

    Buz Barstow

    Insert

    Phosphate ABC transporter ATP binding subunit
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKOS6b-KOpstC

    Plasmid

    #237502

    Purpose

    Knockout of pstC

    Depositor

    Buz Barstow

    Insert

    Phosphate ABC transporter ATP binding subunit pstC
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKOS6b-URP114

    Plasmid

    #237505

    Purpose

    Upregulation of mgdh with P114

    Depositor

    Buz Barstow

    Insert

    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
    

    Expression

    Bacterial

    Mutation

    Upregulation of mgdh with P114

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKOS6b-URtufB

    Plasmid

    #237506

    Purpose

    Upregulation of mgdh with tufB

    Depositor

    Buz Barstow

    Insert

    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
    

    Expression

    Bacterial

    Mutation

    Upregulation of mgdh with tufB

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTargetF

    Plasmid

    #62226

    Purpose

    Constitutive expression of sgRNA without donor editing template DNA

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pij23119

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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