31,004 results

1. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTA-Mob 2.0

   Plasmid

   #149662

   Purpose

   Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

   Depositor

   Bogumil Karas

   Article

   Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Expression

   Bacterial

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p11_AaC60αβ/C20

   Plasmid

   #229506

   Purpose

   Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Chaperonin 60α
   Chaperonin 60β
   Chaperonin 20
   

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCV-Cas9

   Plasmid

   #123643

   Purpose

   Expresses Cas9 with an N-terminal HUH-tag called PCV2

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   (EAAAK)8 linker and His6 + SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29-bdSUMO-Anti-ALFA nanobody

   Plasmid

   #212311

   Purpose

   Expression of anti-ALFA nanobody

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   anti-ALFA nanobody
   

   Tags

   bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS130

   Plasmid

   #26290

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9-PCV

   Plasmid

   #123644

   Purpose

   Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)_ALFA-tag-SNAP

    Plasmid

    #136628

    Purpose

    Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJG103

    Plasmid

    #158533

    Purpose

    wrmScarlet11 x3 tandem repeats

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet11 x3 tandem repeats
    

    Expression

    Bacterial

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNF02-mJuniper

    Plasmid

    #219398

    Purpose

    Constitutive expression of mJuniper in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mJuniper
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p27-mVenus reporter (PB)

    Plasmid

    #190731

    Purpose

    The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    mVenus
    p27
    

    Expression

    Bacterial and Mammalian

    Mutation

    p27-F62A-F64A

    Promoter

    EF1α

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Sfp

    Plasmid

    #159617

    Purpose

    Expression plasmid for Sfp phosphopantetheinyl transferase

    Depositor

    Christopher T Walsh, Jun Yin

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    Sfp
    

    Tags

    6HIS

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCF1135

    Plasmid

    #222106

    Purpose

    E. coli - Veillonella shuttle plasmid with single selectable marker

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sirius/pRSETB

    Plasmid

    #51956

    Purpose

    bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

    Insert

    Sirius
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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