31,004 results

1. pBZS-M.CviPII (NDel15)

   Plasmid

   #198356

   Purpose

   Express M.CviPII (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPII
   

   Expression

   Bacterial

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG 25

   Plasmid

   #63867

   Purpose

   Expression of beta-galactosidase at high levels in E. coli

   Depositor

   Albert Dahlberg

   Article

   O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   lacZ (lacZ Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   PtacI

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pet26-MBP-ELIC

   Plasmid

   #39239

   Depositor

   Raimund Dutzler

   Article

   Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

   Insert

   Eriwinia chrysanthemi - pentameric ligand gated ion channel
   

   Tags

   His-10 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrc-trGPPS(CO)-LS

   Plasmid

   #50603

   Purpose

   pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

   Depositor

   Jay Keasling

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Inserts

   Limonene Synthase
   ApR
   LacIq
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   truncated codon optimized geranylpyrophosphate sy…

   Promoter

   Trc

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15TV-L-AldA WT

   Plasmid

   #187976

   Purpose

   Expresses human aldolase A

   Depositor

   Audrey Lamb

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   AldA WT (ALDOA Human)
   

   Tags

   Hexahistag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1-3xFLAG

   Plasmid

   #129570

   Purpose

   Empty backbone for FLAG tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-3-mR7BD

   Plasmid

   #79149

   Purpose

   bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

   Depositor

   Aimee Edinger

   Article

   Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

   Insert

   R7BD (Rilp Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 220-299

   Promoter

   tac

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-MCM4/6

   Plasmid

   #116951

   Purpose

   Expresses human MCM4 and human MCM6

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM6 (MCM6 Human)
   Human MCM4 (MCM4 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. OmpF

   Plasmid

   #213135

   Purpose

   Expresses outer membrane protein F

   Depositor

   Sebastian Hiller

   Insert

   OmpF (23-362) (ompF E.coli (K12))
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHnCBS1D

    Plasmid

    #52065

    Purpose

    expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

    Depositor

    David Savage

    Article

    Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

    Insert

    CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
    

    Expression

    Bacterial

    Available Since

    April 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-HaloTag-CNA35

    Plasmid

    #215457

    Purpose

    Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    CNA35
    

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Includes stop codon

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    His, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCX

    Plasmid

    #60681

    Purpose

    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression

    Depositor

    David Ackerley

    Article

    Prosser et al Biotechnol Lett. 2014 Sep 26.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIC-EcTOP

    Plasmid

    #224463

    Purpose

    Expression of recombinant Escherichia coli topoisomerase I

    Depositor

    Yuk-Ching Tse-Dinh

    Article

    Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

    Insert

    Escherichia coli TopA
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-Hsp90 N(9-236)

    Plasmid

    #22481

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90 alpha N domain (HSP90AA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB094a

    Plasmid

    #79241

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4; PC araC
    

    Expression

    Bacterial

    Promoter

    PBAD; PC

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTn7xTS_SpyCatcher003:mNG

    Plasmid

    #225190

    Purpose

    Used for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 site

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    SpyCatcher003:mNeonGreen
    

    Tags

    SpyCatcher

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAX1_HS_gpN:SpyTag003

    Plasmid

    #225194

    Purpose

    Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag003
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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