30,998 results

1. 14x PP7 with loxP-kanMX-loxP

   Plasmid

   #189939

   Purpose

   Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

   Depositor

   Tineke Lenstra

   Article

   Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   14x PP7 with loxP-kanMX-loxP
   

   Tags

   kanMX

   Expression

   Bacterial

   Available Since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-PP7v3

   Plasmid

   #107230

   Purpose

   Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   24xPP7v3
   

   Tags

   3x FLAG-tag and P2A site

   Expression

   Bacterial

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

   Plasmid

   #176743

   Purpose

   Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Cx43-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NSUN5

   Plasmid

   #25491

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NSUN5 (NSUN5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ISA431: aTc-MP6

   Plasmid

   #189935

   Purpose

   Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

   Depositor

   Nathan Crook

   Article

   Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

   Insert

   danQ926, dam, seqA, emrR, ugi and cda1
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIS-GB1-BP1

   Plasmid

   #133427

   Purpose

   Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   SREBF1 (SREBF1 Human)
   

   Expression

   Bacterial

   Mutation

   SREBP1a amino acids 17-42 fused to GB1 solubilit…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWLlR5

   Plasmid

   #149492

   Purpose

   Broad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LacI-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLlacO-1

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJOE6227.1

   Plasmid

   #135078

   Purpose

   An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

   Depositor

   Josef Altenbuchner

   Article

   Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

   Insert

   up- and downstream regions of upp (upp Pseudomonas putida)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDual-eGFP(H66)

    Plasmid

    #63558

    Purpose

    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(H66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

    Plasmid

    #72240

    Purpose

    Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    GLT1 GAL1p 24xPP7 V3
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTL032

    Plasmid

    #80928

    Purpose

    14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP

    Depositor

    Daniel Larson

    Article

    Lenstra et al Mol Cell. 2015 Nov 19;60(4):597-610. doi

    Insert

    14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCWU6-xylR-E-luciferase

    Plasmid

    #190721

    Purpose

    fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

    Depositor

    Chenggang Wu

    Article

    Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

    Insert

    xylR-E-luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH6HTC_PRMT1-Halo

    Plasmid

    #175334

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    PRMT1 (PRMT1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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