31,176 results
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Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3
Plasmid#189754PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent proteinDepositorInsertmScarlet3
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST
Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneTagsGSTExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA559
Plasmid#186235PurposepTet-LbuCas13a crRNA entry vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
spNW15
Plasmid#173483PurposeExpresses spNW15 in Bl21DepositorInsertspNW15
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
ExpressionBacterialPromoterN/AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-Streptavidin-K121R
Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-GST-TEV-BRD4(42-459) BD1-BD2
Plasmid#233887PurposepGex for protein overexpression of human BRD4DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
cwby0001
Plasmid#232210PurposeGGA cloning M-AGGA-promoter-ccdb-TTCC-SNAC-HISDepositorInsertccdb
ExpressionBacterialPromoterT7Available SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-pBAD
Plasmid#54622PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis and mEGFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only