30,899 results
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Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32G, E107P, D158T, I159SAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-super TEV protease
Plasmid#171782PurposeExpresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.DepositorInsertMBP-sTEV
TagsArg6 tag and Internal His6 tagExpressionBacterialMutationNo internal TEV cleavage site between the MBP and…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1D1
Plasmid#199768PurposeExpresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1D1
TagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls3-sfGFP
Plasmid#205750PurposeExpresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls3 (Phyperspank mutant, IPTG inducible)Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRExpressionBacterialPromoterPgdh and PxylAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls1-sfGFP
Plasmid#205748PurposeExpresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls1 (Phyperspank mutant, IPTG inducible)Available SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP26
Plasmid#199769PurposeExpresses split-intein fused MSP26 for circularization during expression (internal His-Tag)DepositorInsertcMSP26
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH4-6
Plasmid#199766PurposeExpresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH46
TagsHis6-TagExpressionBacterialMutationinternal His-TagAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-EBFP2
Plasmid#14891PurposeBacterial expression of EBFP2 blue fluorescent proteinDepositorHas ServiceCloning Grade DNAInsertEBFP2
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterhyper-pSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
TagsHis14 and Tev protease cleavage siteExpressionBacterialAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only