34,175 results

Showing: 1861 - 1880 of 34175 results

Results per page:

Sort results by:

pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
p2CT-His-MBP-Lsh_C2c2_WT

Plasmid

#83487

Purpose

Bacterial expression of WT L. shahii C2c2 CRISPR effector

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lsh_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Publication

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pGEX-4T1-RhoA G17A

Plasmid

#69357

Purpose

expression of nucleotide free RhoA in bacterial cells

Depositor

Rafael Garcia-Mata

Publication

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Mutation

G17A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pHYRSF53

Plasmid

#64696

Purpose

His-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, and NpuDnaE-N102

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

Enlarge
pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Publication

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Publication

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Publication

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

6xHis and YPet

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pACλCI

Plasmid

#53730

Purpose

PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

bacteriophage lambda CI protein

Expression

Bacterial

Promoter

lac UV5

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pBRα

Plasmid

#53731

Purpose

Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

Depositor

Ann Hochschild

Publication

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

αNTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pMV306DIG13+FFlucRT

Plasmid

#49998

Purpose

Integrase removed to increase stability of the plasmid in mycobacteria

Depositor

Brian Robertson

Publication

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pTrcHisB-TRF2

Plasmid

#50488

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Publication

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-500

Promoter

pTrc

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pOSIP-CT (CamR, P21)

Plasmid

#45981

Depositor

Drew Endy, Keith Shearwin

Publication

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pGR

Plasmid

#46002

Purpose

Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Publication

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Promoter

Ptac

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pGEXndoS

Plasmid

#44655

Depositor

Mattias Collin

Publication

Collin et al Infect Immun. 2001 Nov . 69(11):7187-9.

Insert

ndoS (ndoS Streptococcus pyogenes)

Tags

Gst

Expression

Bacterial

Mutation

None

Promoter

tac

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pQTEV-LRPAP1

Plasmid

#31327

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

low density lipoprotein receptor-related protein associated (LRPAP1 Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge