4,932 results

1. pKC52

   Plasmid

   #226780

   Purpose

   Expresses Drosophila UNC-104(R348G) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R348G) (unc-104 Fly)
   

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 348 to Glycine

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKC50

   Plasmid

   #226778

   Purpose

   Expresses Drosophila UNC-104(V6M) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (V6M) (unc-104 Fly)
   

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Valine 6 to Methionine

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKC28

   Plasmid

   #226776

   Purpose

   Expresses Drosophila UNC-104(G618R) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (G618R) (unc-104 Fly)
   

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   G618R

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAaePUb::Cas9-2A-Neo

   Plasmid

   #176680

   Purpose

   Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-795

   Plasmid

   #161881

   Purpose

   Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Insect

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-U63-gRNA2.1

   Plasmid

   #170512

   Purpose

   gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-3xP3-GFP

   Plasmid

   #130277

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-GFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFast-Flag-hHus1

   Plasmid

   #222470

   Purpose

   Expresses hHus1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hHus1 (HUS1 Human)
   

   Tags

   Flag epitope

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFast-His6-hRad17

   Plasmid

   #222465

   Purpose

   Expresses hRad17 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad17 (RAD17 Human)
   

   Tags

   His

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIE2-DBD

    Plasmid

    #21602

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    GAL4 DBD (GAL4 Budding Yeast)
    

    Use

    Gateway destination vector

    Tags

    3xHA and Gateway destination cassette

    Expression

    Insect

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. 20XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111546

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1e-CDK12/Cyclin K

    Plasmid

    #231730

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG1e-CDK13/Cyclin K

    Plasmid

    #231731

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK13 (CDK13 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Tags

    mCherry Streptag II

    Expression

    Insect

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 50 pLIB_STREP

    Plasmid

    #160275

    Purpose

    N-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pActP-dtrpA1

    Plasmid

    #64713

    Purpose

    inducing calcium influx in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    dtrpA1 (TrpA1 )
    

    Expression

    Insect

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS26_QUAS_CsChrimson

    Plasmid

    #175548

    Purpose

    Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

    Depositor

    Leslie Vosshall

    Article

    Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

    Insert

    CsChrimson
    

    Tags

    tdTomato

    Expression

    Insect

    Promoter

    QUAS

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDR125

    Plasmid

    #37150

    Depositor

    Dale Ramsden

    Insert

    lig4 (LIG4 Human)
    

    Tags

    H6

    Expression

    Insect

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pF3BGX-T2A-GAL4-DBD

    Plasmid

    #138394

    Purpose

    RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4-DBD
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. act5c-LbCas12a

    Plasmid

    #140620

    Purpose

    Ubiquitous expression of LbCas12a in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Insert

    LbCas12a
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHACK-GAL4>QF2(newHA1)

    Plasmid

    #104873

    Purpose

    HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    T2A-QF2
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAW-Nb127D01-mCherry

    Plasmid

    #171573

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-mCherry (CXCR2 Human)
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Promoter

    osk

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTriEx-mVenus-Zdk1

    Plasmid

    #81047

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Hy_pMT Laccase2 MCS Exon Vector

    Plasmid

    #69884

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc V5 dDispatched WT HA

    Plasmid

    #126405

    Purpose

    Expression of V5 dDispatched WT HA in Drosophila cells lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFlpStop-HDR-UAS-2.1-tdTom

    Plasmid

    #89148

    Purpose

    FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    5XUAS, 3XP3-dsRed

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pE5n

    Plasmid

    #17459

    Purpose

    modified pENTR vector for N-terminal ECFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    ECFP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 78H05-FRT100-STOP-FRT100-p65.AD

    Plasmid

    #232828

    Purpose

    Enhancer 78H05 driven p65.AD, gated by Sparse.FRT100

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT100-STOP-FRT100
    p65.AD
    

    Expression

    Insect

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBP-Sparse.FRT10-p65.AD

    Plasmid

    #232830

    Purpose

    Enhancer-free Sparse.FRT10 p65.AD with ccdB for Gateway

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    p65.AD
    

    Expression

    Insect

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHD-SPARC2-D-LexA::p65

    Plasmid

    #133562

    Purpose

    Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster U6:3

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFastBac LIC cloning vector (4A)

    Plasmid

    #30111

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAC5.1-dCry-V5His

    Plasmid

    #136237

    Purpose

    Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

    Depositor

    Steven Reppert, Aziz Sancar

    Article

    Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

    Insert

    DmCry
    

    Tags

    His and V5

    Expression

    Insect

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. H9 F42A pAcGP67a

    Plasmid

    #41810

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    H9 Super-2 (IL2 Human)
    

    Tags

    8x His

    Expression

    Insect

    Mutation

    Phenylalanine 42 to Alanine

    Promoter

    Polyhedron

    Available Since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only