4,932 results

1. RABEP1A-c019

   Plasmid

   #175477

   Purpose

   Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   RABEP1 (RABEP1 Human)
   

   Tags

   TEV-TwinStrep

   Expression

   Insect

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLfa-PUb-EGFP-3'UTR#13

   Plasmid

   #176338

   Purpose

   The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

   Depositor

   Zach Adelman

   Article

   Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

   Insert

   3'UTR#13
   

   Expression

   Insect

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB1.HMBP.PrS.RBBP4

   Plasmid

   #125164

   Purpose

   Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Histone-binding protein RBBP4 (RBBP4 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCoofy11

   Plasmid

   #44002

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His10

   Expression

   Insect

   Promoter

   hr5

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. PUC19_C_tag

   Plasmid

   #186659

   Purpose

   Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   C-terminal 3xFlag-3xHA Tag
   

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASTLOTattB_VHH-GFP4::CD8::mCherry

   Plasmid

   #163917

   Purpose

   Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

   Depositor

   Markus Affolter

   Article

   Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

   Insert

   Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

   Plasmid

   #190928

   Purpose

   Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Tags

   Strep-TEV-GFP

   Expression

   Insect

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dVGlut-Gal80

   Plasmid

   #52555

   Purpose

   Expresses Gal80 under vesicular glutamate transporter promoter

   Depositor

   Leslie Vosshall

   Insert

   VGlut-Gal80 (VGlut, GAL80 Budding Yeast, Fly)
   

   Expression

   Insect

   Promoter

   Drosophila Synthetic Core Promoter

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD-SPARC2-I-LexA::p65

   Plasmid

   #133563

   Purpose

   Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   20X UAS

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-EPCP5

    Plasmid

    #228879

    Purpose

    Expression of Z-tagged LRRK2 KW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1846

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-CoRESTfl

    Plasmid

    #109156

    Purpose

    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    

    Tags

    3xFLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFL His-p97

    Plasmid

    #113510

    Purpose

    Expression of His-p97 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97 (VCP Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_His-ZZ-TEV-Lis1

    Plasmid

    #132539

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

    Depositor

    Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Tags

    8x His, Linker-TEV-linker_TEV, and ZZ

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLib6.4B-Aalb_743

    Plasmid

    #176676

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOET5 rota-VLP

    Plasmid

    #218147

    Purpose

    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).

    Depositor

    Minna Hankaniemi

    Insert

    Rotavirus VP6
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOET5 CVB3-VLP

    Plasmid

    #218146

    Purpose

    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

    Depositor

    Minna Hankaniemi

    Inserts

    VP1–VP4
    3CD
    

    Expression

    Insect

    Promoter

    CMV and Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOET5 GII.4 Sydney noro-VLP

    Plasmid

    #218092

    Purpose

    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).

    Depositor

    Minna Hankaniemi

    Insert

    noro-VLP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. M2e-noro-VLP

    Plasmid

    #201192

    Purpose

    Expresses noro-VLP decorated with influenza M2e in insect cells

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

    Insert

    M2e-noro-VLP
    

    Tags

    Influenza M2e peptide

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRISPaint-sfGFP-3xP3-RFP

    Plasmid

    #127566

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pE2c

    Plasmid

    #17462

    Purpose

    modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCKFB1

    Plasmid

    #31280

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Clock (Clock Mouse)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

25. p130Cas Y15F

    Plasmid

    #186154

    Purpose

    For expressing recombinant p130Cas in insect cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    BCAR1 (Bcar1 Rat)
    

    Tags

    His6 (Tev)

    Expression

    Insect

    Mutation

    15Y residues mutated to Alanine

    Promoter

    polyhedrin

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BacPAK Myc-S1

    Plasmid

    #19424

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Rpn2 (PSMD1, RPN2 Human)
    

    Tags

    His and Myc

    Expression

    Insect

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pE1n

    Plasmid

    #17441

    Purpose

    modified pENTR vector for N-terminal STREP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    STREP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJFRC-nSyb-IVS-PhiC31

    Plasmid

    #133868

    Purpose

    Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Expression

    Insect

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pUAST-2x attB

    Plasmid

    #206362

    Purpose

    includes two standard UAS cassettes and a third cassette without a promoter

    Depositor

    Erdem Bangi

    Article

    Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRL-ubi63E

    Plasmid

    #74280

    Purpose

    Renilla control plasmid driven by the ubiquitin 63E promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Insert

    Renilla luciferase
    

    Expression

    Insect

    Promoter

    ubiquitin 63E promoter

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJFRC161-20XUAS-IVS-KD::PEST

    Plasmid

    #32140

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    KD::PEST
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUAST-yki:FLAG(3x)

    Plasmid

    #85622

    Purpose

    Express 3x Flagged yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Development. 2008 Mar;135(6):1081-8. doi

    Insert

    yki (yki Fly)
    

    Tags

    3x FLAG

    Expression

    Insect

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. OA-1067B

    Plasmid

    #200252

    Purpose

    pBac-U6-gRNA(doublesex)-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

    Insert

    4 gRNAs targeting Doublesex
    

    Expression

    Insect

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJFRC8-40XUAS-IVS-mCD8::GFP

    Plasmid

    #26221

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQUASp-mCD8mCherry

    Plasmid

    #46164

    Purpose

    Expression of murine CD8mCherry in insects

    Depositor

    Christopher Potter

    Insert

    mCD8mCherry
    

    Expression

    Insect

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OA-986F (AAEL003877-dCas9-VPR)

    Plasmid

    #183993

    Purpose

    Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    AAEL003877-dCAS9-VPR
    

    Use

    CRISPR

    Tags

    dCas9-VPR

    Expression

    Insect

    Promoter

    AAEL003887

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFasBac+GFP-CAMSAP1

    Plasmid

    #59036

    Purpose

    for baculovirus expression of CAMSAP1 in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 (CAMSAP1 Human)
    

    Tags

    His and eGFP

    Expression

    Insect

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge