4,932 results

1. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFB1.HMBP.PrS.dEZH2

   Plasmid

   #220241

   Purpose

   Expression of an EZH2 catalytic site mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   dEZH2 (EZH2 Human)
   

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   Y731F

   Promoter

   Polyhedrin

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. IL2 pAcGP67a

   Plasmid

   #41806

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   IL2 (IL2 Human)
   

   Tags

   6x His

   Expression

   Insect

   Promoter

   Polyhedron

   Available Since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSTARR-seq_fly-hsp70

   Plasmid

   #71500

   Purpose

   flySTARR-seq screening vector with hsp70 core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBP2MF

   Plasmid

   #31279

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

   Insert

   Per2 (Per2 Mouse)
   

   Tags

   Flag and Myc

   Expression

   Insect

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pTV-Gal4DBD2xHighMag1

   Plasmid

   #182023

   Purpose

   Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

   Depositor

   Jean-Paul Vincent

   Article

   di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

   Insert

   Gal4DBD2xHighMag1
   

   Use

   Donor plasmid

   Expression

   Insect

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFBD-Akt1(E17K)

    Plasmid

    #86563

    Purpose

    Expresses human full-length Akt1(E17K) mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    E17K mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINTRX

    Plasmid

    #41116

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. IMPT-2070

    Plasmid

    #172092

    Purpose

    pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

    Depositor

    Raymond Stevens

    Article

    Liu et al Science. 2012 Jul 13;337(6091):232-6. do

    Insert

    Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)
    

    Tags

    cytochrome b562RIL

    Expression

    Insect

    Promoter

    gp64

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. UAS-u(S)Cas9

    Plasmid

    #127383

    Purpose

    Expresses Cas9 at high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(S)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid

    #172915

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7f-T2A-tdTomato
    

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #214139

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Tags

    2xSTII

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQUASp-mCD8GFP

    Plasmid

    #46163

    Purpose

    Expression of murine CD8GFP in insects

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    mCD8GFP
    

    Expression

    Insect

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dynein-2_complex

    Plasmid

    #132536

    Purpose

    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Inserts

    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    

    Tags

    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid

    #22017

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLib6.4-Aalb_743

    Plasmid

    #176662

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p{Mhc-F4-678-eGFP::FRT,attB}

    Plasmid

    #182185

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p{ie1-DsRed::FRT-RC,attB}

    Plasmid

    #182191

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. R979-X58-008: Hs.KRAS4b(1-188)

    Plasmid

    #159691

    Purpose

    Baculovirus protein expression of KRAS4b (1-188)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

    Insert

    Hs.KRAS4b(1-188) (KRAS Human)
    

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKM50

    Plasmid

    #123656

    Purpose

    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Aedes aegypti UbL40 promoter
    

    Use

    Luciferase

    Expression

    Insect

    Promoter

    UbL40 promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2Bac.His.XPB

    Plasmid

    #39216

    Depositor

    Aziz Sancar

    Insert

    XPB (ERCC3 Human)
    

    Tags

    His

    Expression

    Insect

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pJFRC151-3XUAS-IVS-Flp2::PEST

    Plasmid

    #32133

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase2::PEST
    

    Tags

    PEST

    Expression

    Insect

    Mutation

    Glycine at Amino Acid 5

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

    Plasmid

    #78509

    Purpose

    Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD45R0Ex (PTPRC Human)
    

    Tags

    His10 and SNAPf

    Expression

    Insect

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pTriEx-NTOM20-mVenus-Zdk3

    Plasmid

    #81012

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk3
    

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p2Bac.His.XPD

    Plasmid

    #39217

    Depositor

    Aziz Sancar

    Insert

    XPD (ERCC2 Human)
    

    Tags

    His (HHYHHH)

    Expression

    Insect

    Mutation

    XPD starts at aa4

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CR7T

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFBD-ESCRT 0

    Plasmid

    #21499

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)
    

    Tags

    GST and His-MBP

    Expression

    Insect

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AlphaC

    Plasmid

    #124525

    Purpose

    pColE1_AlphaC

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pExpreS2-1

    Plasmid

    #175360

    Purpose

    ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJFRC-20X-UAS-ADARcd

    Plasmid

    #81173

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    UAS-ADARcd
    

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid

    #173674

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBAC-DsRed-QF2-hsp70

    Plasmid

    #104876

    Purpose

    General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge