5,038 results
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Plasmid#63752PurposeExpresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only
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pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-tubulin-IVS-Syn21-QSco-p10
Plasmid#46132DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx6 WT
Plasmid#53479DepositorAvailable SinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 4x
Plasmid#20930DepositorInsert4x let-7 target sites
UseLuciferaseExpressionInsect and MammalianAvailable SinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
AT5G63930 X059_pECIA2
Plasmid#115116PurposeBait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.DepositorInsertAT5G63930 (AT5G63930 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G17230 X074_pECIA2
Plasmid#115096PurposeBait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.DepositorInsertAT1G17230 (AT1G17230 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G33170 X049_pECIA2
Plasmid#115101PurposeBait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.DepositorInsertAT2G33170 (AT2G33170 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G74360 X038_pECIA2
Plasmid#115080PurposeBait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.DepositorInsertAT1G74360 (AT1G74360 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AT5G63930 X059_pECIA14
Plasmid#114916PurposePrey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.DepositorInsertAT5G63930 (AT5G63930 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G17230 X074_pECIA14
Plasmid#114896PurposePrey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.DepositorInsertAT1G17230 (AT1G17230 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G33170 X049_pECIA14
Plasmid#114901PurposePrey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.DepositorInsertAT2G33170 (AT2G33170 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G74360 X038_pECIA14
Plasmid#114880PurposePrey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.DepositorInsertAT1G74360 (AT1G74360 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_774
Plasmid#176659PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB-609 U6-sgRNA1 ATCC GGAA
Plasmid#173671PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHP1048 - pFB-RSP5
Plasmid#32434DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6c-T
Plasmid#117211PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6c promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
ExpressionInsectAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBac-GH146-QF
Plasmid#24353DepositorInsertQF
TagsdsRedExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217513PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>myr::4xSNAPf
Plasmid#87639PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>myr::4xSNAPfDepositorInsertmyr::4xSNAPf
ExpressionInsectAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6
Plasmid#224346PurposeExpresses human KDAC6 (HDAC6) in insect cellsDepositorAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPaint-T2A-QF2-3xP3-RFP
Plasmid#127559PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.DepositorInsertQF2
UseCRISPRExpressionInsectAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Flp1)
Plasmid#194770PurposeTo insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.DepositorInsertT2A-Flp1
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-DsRed::GW,attB}
Plasmid#182173Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-eGFP::GW,attB}
Plasmid#182172Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInserteGFP
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-DsRed::GW,attB}
Plasmid#182171Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-FLPo
Plasmid#24357DepositorInsertFLPo
ExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_eGFP::Dpp
Plasmid#163702PurposeGFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancersDepositorAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD-3xP3-dsRed-DattP
Plasmid#133559PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructsDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_ANP
Plasmid#133243PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertrat atrial natriuretic peptide-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-C450A
Plasmid#22021PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV domain contains the light-insensitive C450A m…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]
Plasmid#71809PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
OA-1043
Plasmid#164586Purposeclone in U6 and DRDepositorInsertU6 and DR
ExpressionInsectAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-Flag-RELISH
Plasmid#63750PurposeExpresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-MAL-SYK
Plasmid#157990PurposeExpresses SYK in insect cell (sf9)DepositorInsertSpleen tyrosine kinase (SYK Human)
TagsMet-Ala-Leu and add Leu,Glu residues +His tagExpressionInsectAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AByG
Plasmid#111083PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInserts3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right
ExpressionInsectAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
Plasmid#71811PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.dEZH2
Plasmid#220241PurposeExpression of an EZH2 catalytic site mutant in insect cells.DepositorAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pXL-BACII-LoxP-3xPDsRed-LoxP
Plasmid#26852DepositorInsertLoxP-3xPDsRed-LoxP
ExpressionInsectAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only