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4,958 results

Showing: 1051 - 1100 of 4958 results

  3. pOCC61

    Plasmid
    #118877
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118877-plasmid-map-sequence-id-232346

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  10. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Tags
    2xSTII
    Expression
    Insect
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  13. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Expression
    Insect
    Promoter
    act5C
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62211-plasmid-map-sequence-id-101870

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  20. pTriEx-mVenus-PA-Rac1-C450A

    Plasmid
    #22021
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV domain contains the light-insensitive C450A m…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  21. pAttB-Tub

    Plasmid
    #203966
    Purpose
    Fly transformation vector with Tubulin promoter and SV40 PA site and a UTR for ubiquitous expression
    Depositor
    Andreas Jenny
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203966-plasmid-map-sequence-id-403023

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  25. pHACK-GAL4>GAL80

    Plasmid
    #104874
    Purpose
    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.
    Depositor
    Christopher Potter
    Insert
    T2A-GAL80
    Use
    CRISPR
    Expression
    Insect
    Available Since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104874-plasmid-map-sequence-id-204699

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  27. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pUAST tdTomato Profilin WT

    Plasmid
    #53509
    Depositor
    Matthew Scott
    Insert
    profilin (chic Fly)
    Tags
    tdtomato
    Expression
    Insect
    Promoter
    GAL4
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pAc5.1B-HA-SBP_J

    Plasmid
    #146621
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146621-plasmid-map-sequence-id-421635

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  33. pAc5.1B-V5-SBP_J

    Plasmid
    #146633
    Purpose
    Insect Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146633-plasmid-map-sequence-id-421638

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  42. VanGlow-RR

    Plasmid
    #83343
    Purpose
    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Available Since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83343-plasmid-map-sequence-id-158672

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  45. AAEL007097-Cas9

    Plasmid
    #100705
    Purpose
    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL007097 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100705-plasmid-map-sequence-id-193542

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  46. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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Showing: 1051 - 1100 of 4958 results