4,932 results
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Plasmid#127559PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.DepositorInsertQF2
UseCRISPRExpressionInsectAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
TagsV5ExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3X-UASTattB
Plasmid#189859PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
ExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AByG
Plasmid#111083PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInserts3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right
ExpressionInsectAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-Flag-RELISH
Plasmid#63750PurposeExpresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_ANP
Plasmid#133243PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertrat atrial natriuretic peptide-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS1
Plasmid#60784PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS1
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pattB-QF-sv40
Plasmid#24367DepositorInsertQF
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2 D597N
Plasmid#136236PurposeExpressing catalytically inactive human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
TagsSterp SumoExpressionInsectMutationD597NPromoterpolyhedrinAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>mCD8-GFP
Plasmid#24361DepositorInsertmCD8-GFP
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]
Plasmid#71809PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
10XUAS-flp-DD
Plasmid#124777PurposeExpresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized FLP (FLP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusExpressionInsectPromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2Available SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-623 (attR4-attR3)
Plasmid#161878PurposeMultisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D WT
Plasmid#52242PurposeDrosophila expression of YFP-Ras85DDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LHP1048 - pFB-RSP5
Plasmid#32434DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_774
Plasmid#176659PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-tubulin-IVS-Syn21-QSco-p10
Plasmid#46132DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
AlphaB
Plasmid#124524PurposepColE1_AlphaBDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlphaD
Plasmid#124526PurposepColE1_AlphaDDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsGFP-His8ExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Cdc42
Plasmid#101681Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et alDepositorInsertCdc42
Tags6xHis and FRET Antenna (mCerulean-cpVenus)ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSB-610 U6-sgRNA2 GGAA AGAG
Plasmid#173672PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-RLuc-A90-HhR_O
Plasmid#147073PurposeInsect Expression of A90-HhRDepositorInsertA90-HhR
ExpressionInsectMutationA127 instead of A90 (see comments below)Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Agam_695
Plasmid#176654PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-7m1-QFBDADM1-hsp70
Plasmid#46116DepositorInsertQF#7m1
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK23
Plasmid#72851Purposetemplate for C-terminal eGFP tagging, Puromycin-resistanceDepositorInserteGFP
ExpressionInsectPromotercopiaAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159553PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22 / PP1gamma
Plasmid#113511PurposeExpression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DPP3
Plasmid#25154PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC119
Plasmid#118884Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mGFP, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only